Kuno Akihiro

クノアキヒロ | Kuno Akihiro

Affiliation and department：
Homepage URL (1)： https://www.md.tsukuba.ac.jp/LabAnimalResCNT/mouse-meets-computer/

Research field (3)： Systems genomics , Genomics , Laboratory animal science

Research keywords (4)： Bioinformatics , Genome biology , Genome editing , Laboratory animal science

Research theme for competitive and other funds (9)：

2024 - 2028 Development of precise genome engineering methods using modiied CRISPR-Cas3
2024 - 2026 ゲノム編集動物の標的座位遺伝型を超高精度で決定するnCATS-DAJIN法の開発
2023 - 2026 Cell fate decision by dose of transcription factors
2022 - 2025 新規direct-cKOシステムでの必須遺伝子探索とその網羅的解析基盤の構築
2020 - 2024 Biological functions of alternative splicing on mouse gastrulation

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Papers (38)：

Taito Taki, Kento Morimoto, Seiya Mizuno, Akihiro Kuno. KOnezumi-AID: A Software to Automate the Design of Multiplex Knockouts Using Target-AID. bioRxiv. 2024
Yoshimi K, Kuno A, Yamauchi Y, Hattori K, Taniguchi H, Mikamo K, Iida R, Ishida S, Goto M, Takeshita K, et al. Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Cell reports methods. 2024
Manoj Kumar Yadav, Megumi Ishida, Natalia Gogoleva, Ching-Wei Liao, Filiani Natalia Salim, Maho Kanai, Akihiro Kuno, Takuto Hayashi, Zeynab Javanfekr Shahri, Kaushalya Kulathunga, et al. MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue. Cell reports. 2024. 43. 4. 113978-113978
Kento Morimoto, Hayate Suzuki, Akihiro Kuno, Yoko Daitoku, Yoko Tanimoto, Kanako Kato, Kazuya Murata, Fumihiro Sugiyama, Seiya Mizuno. Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions. Open Biology. 2024. 14. 4
Akihiro Kuno. cstag and cstag-cli: tools for manipulating and visualizing cs tags. Journal of Open Source Software. 2024

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Lectures and oral presentations (18)：

Development of DAJIN2 for deciphering complex genotypes arising from CRISPR-Cas genome-editing.
(The 9th Annual Meeting of the Japanese Society for Genome Editing 2024)
EFFECTIVENESS OF IMATINIB FOR MCTO NEPHROPATHY
(60th ERA Congress)
Effectiveness of Imatinib Formcto Nephropathy
(60th ERA Congress)
主な実験マウスデータベースの最近の進展とその情報学的利用
(JAX in Japanウェビナー2023 2023)
速筋線維の形成に不可欠な新規制御因子である大Maf転写因子の機能解析
(日本分子生物学会年会 2022)

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Education (2)：

2013 - 2018 Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba
2007 - 2013 University of Tsukuba School of Medicine Department of Medicine

Professional career (1)：

Work history (3)：

2024/04 - 現在 University of Tsukuba Laboratory Animal Resource Center Researcher
2018/05 - 2024/03 University of Tsukuba Department of Anatomy and Embryology, Assistant Professor
2019/06 - 2021/03 School of Integrative and Global Majors, University of Tsukuba Ph.D. Program in Human Biology Assistant Professor

Committee career (5)：

2023/04 - 2024/03 筑波大学医学群医学類 CBTサイトマネージャー
2021/04 - 2024/03 Ph.D. Program in Human Biology, University of Tsukuba Education committee
2020/04 - 2024/03 Ph.D. Program in Human Biology, University of Tsukuba Admissions and enrollment committee
2020/04 - 2024/03 Ph. D. Program in Humanics, University of Tsukuba Student support committee
2020/04 - 2024/03 Ph.D. Program in Human Biology, University of Tsukuba Student support committee / Vice chairperson

Awards (4)：

2022/11 - 筑波大学医学医療系教員業績評価優秀教員賞
2022/11 - University of Tsukuba Young Investigator Awards
2022/06 - Poster award DAJIN: Validate multiallelic genome editing outcomes using AI-assisted long-read sequencing
2019/09 - Poster award Deep-learning based genotype identifier for genetically modified animals using long DNA sequencer

Association Membership(s) (1)：

The Japanese Society for Genome Editing

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