Kumakura Naoyoshi

クマクラナオヨシ | Kumakura Naoyoshi

Affiliation and department：

Research field (2)： Applied molecular and cellular biology , Conservation science (plants)

Research keywords (7)： Colletotrichum fungi , Plant fungal pathogen , Plant immunity , Genome editing by CRISPR-Cas9 , Secondary metabolites , RNA , virulence factor

Research theme for competitive and other funds (12)：

2024 - 2028 Playing with Appressoria: Advancing Technologies for the Control and Understanding of High Turgor Pressure and Adhesion Force
2023 - 2026 植物病原糸状菌感染に寄与する非リボソームペプチド合成酵素の生産物同定と機能解明
2023 - 2025 Establishment of biochemical reaction system and inhibitor screening of a pair of secondary metabolite-related enzymes that are essential for infection of plant pathogenic fungi
2023 - 2024 Elucidation of infection mechanism using a multiplex gene disruption method (Acceleration phase)
2022 - 2023 植物病原糸状菌付着器の高い膨圧発生を可能にする細胞壁の解析

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Papers (17)：

Naoyoshi Kumakura, Takayuki Motoyama, Keisuke Miyazawa, Toshihiko Nogawa, Katsuma Yonehara, Kaori Sakai, Nobuaki Ishihama, Kaisei Matsumori, Pamela Gan, Hiroyuki Koshino, et al. Dihydroxyhexanoic acid biosynthesis controls turgor in pathogenic fungi. bioRxiv. 2024
Naoyoshi Kumakura, Suthitar Singkaravanit-Ogawa, Pamela Gan, Ayako Tsushima, Nobuaki Ishihama, Shunsuke Watanabe, Mitsunori Seo, Shintaro Iwasaki, Mari Narusaka, Yoshihiro Narusaka, et al. Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses. The New Phytologist. 2024. 242. 1. 170-191
Katsuma Yonehara, Naoyoshi Kumakura, Takayuki Motoyama, Nobuaki Ishihama, Jean-Félix Dallery, Richard O'Connell, Ken Shirasu. Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3-based marker recycling. Molecular Plant Pathology. 2023. 24. 11. 1451-1464
Mingming Chen, Naoyoshi Kumakura (co-first author), Hironori Saito, Ryan Muller, Madoka Nishimoto, Mari Mito, Pamela Gan, Nicholas T Ingolia, Ken Shirasu, Takuhiro Ito, et al. A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants. eLife. 2023. 12
Jinlian Chen, Yoshihiro Inoue, Naoyoshi Kumakura, Kazuyuki Mise, Ken Shirasu, Yoshitaka Takano. Comparative transient expression analyses on two conserved effectors of Colletotrichum orbiculare reveal their distinct cell death-inducing activities between Nicotiana benthamiana and melon. Molecular Plant Pathology. 2021. 22. 8. 1006-1013

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MISC (34)：

熊倉直祐, SINGKARAVANIT-OGAWA Ogawa, GAN Pamela, 津島綾子, 石濱伸明, 渡邊俊介, 瀬尾光範, 岩崎信太郎, 鳴坂真理, 鳴坂義弘, et al. Ribonuclease effectors of Colletotrichum orbiculare potentiate host immune responses. 日本植物病理学会大会プログラム・講演要旨予稿集. 2024. 2024
宮澤佳甫, 熊倉直祐, 松森海晴, 白須賢, 福間剛士. ウリ類炭疽病菌の付着器の細胞壁のナノスケール構造計測. 2023年第84回応用物理学会秋季学術講演会. 2023
Establishment of a CRISPR-Cas9-mediated marker recycling system for multiple-gene disruptions in Colletotrichum higginsianum. 日本植物病理学会大会プログラム・講演要旨予稿集. 2023. 2023
AFM measurement of turgor pressure of an appressorium of Colletotrichum orbiculare. 応用物理学会春季学術講演会講演予稿集(CD-ROM). 2023. 70th
A parasitic fungus employs mutated eIF4A for the survival on rocaglate-synthesizing Aglaia plant. 2022. 2022

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Lectures and oral presentations (8)：

Dihydroxyhexanoic acid biosynthesis controls turgor in pathogenic fungi
(2024 KSPP Fall International Conference 2024)
Plant-fungus arms race mediated by plant secondary metabolites
(21st Conference of Fungal Molecular Biology Society of Japan 2022)
Bottlenecks of phytopathogenic fungal studies
(2022)
Identification of secondary metabolite synthesis genes involved in virulence of Colletotrichum fungi using a multiplex gene disruption system
(Seminar at BIOGER in INRAE, Thiverval-Grignon, France 2021)
Identification of Colletotrichum effectors using comparative genomics and a novel multiplex mutagenesis technique
(Kochi University Department of Life and Environmental Medicine Seminar 2019)

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Education (4)：

2010 - 2014 The University of Tokyo Graduate School of Arts and Sciences Multidisciplinary Sciences, Doctor course
2008 - 2010 The University of Tokyo Graduate School of Arts and Sciences Multidisciplinary Sciences, Master course
2006 - 2008 The University of Tokyo College of Arts and Sciences
2004 - 2006 The University of Tokyo College of Arts and Sciences Natural Science I

Professional career (1)：

Ph. D. (The University of Tokyo)

Work history (9)：

2024/10 - 現在 Japan Science and Technology Agency Presto Sakigake researcher
2020/04 - 現在 RIKEN Center for Sustainable Resource Science Research scientist
2020/12 - 2024/03 Japan Science and Technology Agency ACT-X researcher
2021/07 - 2021/09 INRAE BIOGER Visiting scientist
2017/04 - 2020/03 RIKEN Plant Immunity Research Group, Center for Sustainable Resource Science Postdoc (SPDR fellow)

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Committee career (1)：

2016/03 - 2018/03 The Japanese Society of Young Plant Physiologist Japanese Society of Plant Physiologists

Awards (3)：

2023/09 - The 22nd Applied Physics Society Poster Award Japan Society of Applied Physics Autumn Meeting 2023 Measurement of the nanoscale structure of the cell wall in appressoria of Colletotrichum orbiculare
2012/08 - The XV International Congress of Molecular Plant-Microbe Interactions Wiley Best Poster Award
2010/03 - The University of Tokyo Incentive Award, Graduate School of Arts and Sciences

Association Membership(s) (2)：

THE PHYTOPATHOLOGICAL SOCIETY OF JAPAN , THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

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