Harada Kazuki

Affiliation and department：
Job title：助教

Research field (1)： Animals: biochemistry, physiology, behavioral science

Research keywords (5)： Fluorescent protein , Glucagon-like peptide-1 , Enteroendocrine cell , Hormone secretion , Imaging

Research theme for competitive and other funds (13)：

2023 - 2027 腸内細菌代謝物による認知的柔軟性制御における消化管ホルモンを介した分子機構の解析
2023 - 2026 ホルモン分泌における環状ヌクレオチドシグナリングを照らすキナーゼ光スイッチ開発
2022 - 2024 腸内細菌代謝物が消化管ホルモンを介して行動を制御するシグナル伝達経路の解析
2022 - 2023 粉末ミルクによるグルカゴン様ペプチド-1分泌増強機構の解明
2020 - 2023 Regulation of incretin secretion by microbiota metabolites

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Papers (29)：

Saki Tsuno, Kazuki Harada, Mina Horikoshi, Marie Mita, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi. Mitochondrial ATP concentration decreases immediately after glucose administration to glucose-deprived hepatocytes. FEBS open bio. 2024. 14. 1. 79-95
Mina Horikoshi, Kazuki Harada, Saki Tsuno, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Shin Terada, Takashi Tsuboi. Distinct lactate metabolism between hepatocytes and myotubes revealed by live cell imaging with genetically encoded indicators. Biochemical and biophysical research communications. 2023. 694. 149416-149416
Kazuki Harada, Maoko Takashima, Tetsuya Kitaguchi, Takashi Tsuboi. F-actin determines the time-dependent shift in docking dynamics of glucagon-like peptide-1 granules upon stimulation of secretion. FEBS Letters. 2023
Kazuki Harada, Maoko Takashima, Tetsuya Kitaguchi, Takashi Tsuboi. Exocytotic dynamics of glucagon-like peptide-1 from enteroendocrine L cell line is regulated by actin polymerization. biorxiv. 2022
Mai Takizawa, Yuri Osuga, Rika Ishida, Marie Mita, Kazuki Harada, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi. Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging. Communications Biology. 2022. 5. 1

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MISC (1)：

原田一貴, 和田英治, 清水稀惠, 清水稀惠, 宮崎雅雄, 林由起子, 中村和昭, 坪井貴司. Gut microbiota-mediated regulation of gut hormone secretion and metabolic homeostasis. 日本分子生物学会年会プログラム・要旨集(Web). 2023. 46th

Lectures and oral presentations (1)：

Elucidation of cellular functions by fluorescence imaging
(Symposium for Young Researchers in Behavioral Neuroendocrinology, KOUDOU2017 2017)

Education (3)：

2014 - 2019 The University of Tokyo Graduate School of Arts and Sciences Department of Life Sciences
2012 - 2014 The University of Tokyo College of Arts and Sciences Department of Life Sciences
2010 - 2012 The University of Tokyo College of Arts and Sciences Department of Law

Professional career (1)：

博士(学術) (東京大学)

Work history (3)：

2023/04 - 現在 Meiji University School of Agriculture Department of Life Sciences
2019/04 - 現在 The University of Tokyo Department of Life Sciences, Graduate School of Arts and Sciences Assistant professor
2016/04 - 2019/03 Japan Society for the Promotion of Science Research fellow (DC1)

Awards (9)：

2023/03 - 日本生理学会 2022年度入澤宏・彩記念若手研究奨励賞
2022/06 - 東京大学第21回東京大学生命科学シンポジウム優秀ポスター賞
2019/03 - The University of Tokyo The First National High School Memorial Award 2019
2019/03 - The University of Tokyo President's Award 2019 Coaching activity in The University of Tokyo Secondary School attached to the Faculty of Education
2018/12 - Japanese Association for Science Communication Best Presentation Award for the 7th Annual Meeting of Japanese Association for Science Communication

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Association Membership(s) (4)：

Japanese Association of Science Communication , THE JAPAN NEUROSCIENCE SOCIETY , THE MOLECULAR BIOLOGY SOCIETY OF JAPAN , THE PHYSIOLOGICAL SOCIETY OF JAPAN

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