Rchr
J-GLOBAL ID:201901018057015944   Update date: Feb. 01, 2024

Ishikawa Ken

イシカワ ケン | Ishikawa Ken
Affiliation and department:
Homepage URL  (1): https://www.kurume-u.ac.jp/site/lifescience/top-page.html
Research field  (5): Molecular biology ,  Chemical biology ,  Genomics ,  Genetics ,  Functional biochemistry
Research keywords  (5): CRISPRi ,  CRISPR-Cas9 ,  DNA切断酵素 ,  ゲノム安定性 ,  メタボライト
Research theme for competitive and other funds  (8):
  • 2022 - 2025 Elucidation of cancer mutagen by novel signature analysis
  • 2023 - 2024 分裂酵母の必須遺伝子ノックダウンライブラリ構築とその応用
  • 2022 - 2023 CRISPRiによる分裂酵母の必須遺伝子ノックダウンライブラリ構築
  • 2021 - 2023 細胞外ヘキソース濃度変化に対する細胞適応の仕組み
  • 2021 - 2022 革新的な転写抑制医薬品の開発~dCas9タンパク質の人工進化~
Show all
Papers (16):
  • Ken Ishikawa, Saeko Soejima, Shigeaki Saitoh. Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe. Journal of Cell Science. 2023. 136. 9. jcs261186
  • Ken Ishikawa, Shigeaki Saitoh. Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast. Biomolecules. 2023. 13. 4. 716-716
  • Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe. G3: Genes | Genomes | Genetics. 2021
  • Ken Ishikawa, Scott H. Medina, Joel P. Schneider, Amar J. S. Klar. Glycan Alteration Imparts Cellular Resistance to a Membrane-Lytic Anticancer Peptide. CELL CHEMICAL BIOLOGY. 2017. 24. 2. 149-158
  • Yingbiao Zhang, Tomoyuki Matsuzaka, Hirokazu Yano, Yoshikazu Furuta, Toshiaki Nakano, Ken Ishikawa, Masaki Fukuyo, Noriko Takahashi, Yutaka Suzuki, Sumio Sugano, et al. Restriction glycosylases: involvement of endonuclease activities in the restriction process. NUCLEIC ACIDS RESEARCH. 2017. 45. 3. 1392-1403
more...
MISC (3):
  • Yingbiao Zhang, Tomoyuki Matsuzaka, Hirokazu Yano, Toshiaki Nakano, Ken Ishikawa, Masaki Fukuyo, Noriko Takahashi, Yutaka Suzuki, Sumio Sugano, Hiroshi Ide, et al. Restriction glycosylases: involvement of endonuclease activities in the restriction process. GENES & GENETIC SYSTEMS. 2016. 91. 6. 369-369
  • Ken Ishikawa, Naofumi Handa, Ichizo Kobayashi. Cleavage of a model DNA replication fork by restriction enzymes in vitro. GENES & GENETIC SYSTEMS. 2009. 84. 6. 433-433
  • Ken Ishikawa, Naofumi Handa, Ichizo Kobayashi. Coupling of DNA replication and restriction: A type I restriction enzyme cleaves branched DNAs mimicing replication forks, in vitro. GENES & GENETIC SYSTEMS. 2008. 83. 6. 510-510
Patents (2):
  • 新規制限酵素PabI
  • 無細胞タンパク質合成系を用いる新規ヌクレアーゼのスクリーニング方法
Lectures and oral presentations  (11):
  • A pilot library of essential gene knockdown strains and plasmids for the fission yeast.
    (2023)
  • CRISPRiを用いた分裂酵母の全必須遺伝子ノックダウンライブラリ構築への取り組み
    (第45回分子生物学会年会 2022)
  • 分裂酵母とヒト間で進化的に保存された未解析必須遺伝子のCRISPRiによるノックダウン
    (日本遺伝学会 第94回大会 2022)
  • 分裂酵母におけるCRISPRiを用いた遺伝子ノックダウン
    (第44回分子生物学会年会 2021)
  • 分裂酵母Schizosaccharomyces pombeにおけるdCas9を用いたCRISPRi
    (第73回日本細胞生物学会大会 2021)
more...
Education (3):
  • 2004 - 2009 東京大学大学院 理学系研究科 生物化学専攻 博士課程(小林一三 教授)
  • 2002 - 2004 東京大学大学院 理学系研究科 生物化学専攻 修士課程(小林一三 教授)
  • 1998 - 2002 Tohoku University Faculty of Science Department of Chemistry
Professional career (1):
  • 博士(理学) (東京大学)
Work history (4):
  • 2018/10 - 現在 Kurume University Institute of Life Science
  • 2016/03 - 2018/09 NIH, NCI-Frederick, Chemical Biology Laboratory (Dr. Joel Schneider), Research Fellow
  • 2011/03 - 2016/02 NIH, NCI-Frederick, Gene Regulation and Chromosome Biology Laboratory (Dr. Amar Klar), Visiting Fellow
  • 2009/04 - 2011/02 The University of Tokyo
Association Membership(s) (3):
日本細胞生物学会 ,  THE MOLECULAR BIOLOGY SOCIETY OF JAPAN ,  The Genetics Society of Japan
※ Researcher’s information displayed in J-GLOBAL is based on the information registered in researchmap. For details, see here.

Return to Previous Page