Ohtsubo Yoshiyuki

オオツボヨシユキ | Ohtsubo Yoshiyuki

Affiliation and department：
Job title： Associate Professor

Research field (2)： Applied microbiology , Software

Research keywords (6)：ゲノム , バイオレメディエーション , PCB , カタボライト調節 , 遺伝子発現制御 , 細菌

Research theme for competitive and other funds (22)：

2021 - 2026 土壌微生物機能発揮の鍵となる群集・メタゲノム構造の特定
2022 - 2025 Advanced utilization strategies of beneficial bacteria based on the survival and growth mechanisms of bacteria under poor conditions
2022 - 2024 Approach to the substance of bacterial extracellular genetic information storage device
2019 - 2022 Development of practical use of useful bacteria by understanding their behavior in the environment
2017 - 2020 Soil-derived bacterial consortium capable of degrading chemical pollutant: interaction between pollutant-degrader and co-residing non-degraders

2016 - 2018 Behavior of environmental bacteria in microbial community and oligotrophic growth state

2015 - 2018 Understanding of PCB-degrading soil bacterium and development of experimental methods for applied microbiology

2014 - 2016 Studies on metabolic pathway involved in oligotrophic growth of heterotrophic bacteria

2013 - 2016 Mechanisms for acquiring ability to degrade novel compounds in sphingomonads and their applications

2013 - 2016 Behavior of microbial community in the environment polluted by aromatic compound

2012 - 2014 Studies on oligotrophic mutants of an aerobic heterotrophic bacterium

2011 - 2013 Studies on features and frequencies of recombination in bacterial evolution

2010 - 2012 Studies on molecular mechanisms for functional evolution of bacteria in the environment

2010 - 2012 Response of soil microbial community to exposure of environmental pollutants

2010 - 2011 Genomic organization and genomic dynamism of versatile sphingomonads

2008 - 2010 Revealing catabolite-control mechanisms of soil bacteria

2007 - 2008 挿入配列の土壌環境での動態の解明と新規遺伝子獲得への応用

2006 - 2008 Comprehensive development of bacterial ability for the degradation ofchlorinated environmental pollutants using unexplored genetic sources

2006 - 2008 Horizontal transfer of bacterial genes for degradation of environmental pollutants

2006 - 2007 環境細菌のカタボライト調節分子メカニズムの解明

2004 - 2005 PCB分解土壌細菌のカタボライト調節メカニズムの解明

1998 - 2000 細菌由来のPCB分解遺伝子群の制御因子の解析および有効なPCB生分解系の構築

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Papers (126)：

Satoshi Matsumoto, Kouhei Kishida, Shouta Nonoyama, Keiichiro Sakai, Masataka Tsuda, Yuji Nagata, Yoshiyuki Ohtsubo. Evolution of the Tn4371 ICE family: traR-mediated coordination of cargo gene upregulation and horizontal transfer. Microbiology spectrum. 2024. e0060724
Wenhao Deng, Yoshinobu Takada, Yoshihiko Nanasato, Kouhei Kishida, Leonardo Stari, Yoshiyuki Ohtsubo, Yutaka Tabei, Masao Watanabe, Yuji Nagata. Transgenic Arabidopsis thaliana plants expressing bacterial γ-hexachlorocyclohexane dehydrochlorinase LinA. BMC Biotechnology. 2024. 24. 1
Rie Tateishi, Natsumi Ogawa-Kishida, Nobuharu Fujii, Yuji Nagata, Yoshiyuki Ohtsubo, Shota Sasaki, Keisuke Takashima, Toshiro Kaneko, Atsushi Higashitani. Increase of secondary metabolites in sweet basil (Ocimum basilicum L.) leaves by exposure to N2O5 with plasma technology. Scientific Reports. 2024. 14. 1
Yoshiyuki Ohtsubo, Syoutaro Kawahara, Yuji Nagata. Clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis by Taq polymerase involves nick-translation. Scientific reports. 2024. 14. 1. 2030-2030
Keiichiro Sakai, Kouhei Kishida, Satoshi Matsumoto, Yuji Nagata, Masataka Tsuda, Yoshiyuki Ohtsubo. Three distinct metabolic phases of PCB/biphenyl degrader Acidovorax sp. KKS102 in nutrient broth. Bioscience, biotechnology, and biochemistry. 2024

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MISC (80)：

Why do we need desalting after the Sanger sequence reaction?でどう?. News Letter of the Society of Genome Microbiology, Japan. 2022. 25. 20-21
広瀬侑, 大坪嘉行, 三澤直美, 米川千夏, 長尾信義, 志村遥平, 藤澤貴智, 兼崎友, 加藤浩, 片山光徳, et al. NIESコレクションのシアノバクテリアのゲノム解析. 日本ゲノム微生物学会年会要旨集. 2022. 16th (Web)
大坪嘉行. ゲノム DNA を鋳型にしたサンガーシーケンシング. 日本ゲノム微生物学会ニュースレター. 2021. 24
大坪嘉行. イルミナシーケンスライブラリー、ハイブリッド型のススメ. 日本ゲノム微生物学会ニュースレター. 2021. 23
大坪嘉行. 実験技術紹介コーナー(ニュースレター20号). 日本ゲノム微生物学会ニュースレター. 2019. 20

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Patents (1)：

二本鎖DNA末端の加工方法

Books (1)：

Causes and Solutions of Science and Technology Decline in Japan
2022 ISBN:9798428260090

Lectures and oral presentations (1)：

共創:微生物エコシステム構成原理土壌細菌叢変動とレジームシフト
(第89回日本細菌学会総会 2016)

Works (14)：

QR-code based Attendance Management System "QR AMS"
Yoshiyuki Ohtsubo 2023 - 現在
DNA配列、アミノ酸配列の相互比較描画ソフトウエア「SeqView」
大坪嘉行 2021 - 現在
研究機器共用利用促進システム「DeviceFace」
大坪嘉行 2020 - 現在
シーケンサーウエブ使用簿システム
大坪嘉行 2018 -
シーケンサー予約システム
大坪嘉行 2018 -

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Education (2)：

- 2001 The University of Tokyo Graduate School of Agricultural and Life Sciences
- 1996 The University of Tokyo Faculty of Agriculture

Professional career (1)：

博士(農学) (The University of Tokyo)

Work history (1)：

2001/04 - 2003/03 理化学研究所基礎科学特別研究員

Committee career (5)：

2017/04 - 現在日本農芸化学会化学と生物編集委員
2013/04 - 現在ゲノム微生物学会ニュースレター編集幹事
2013/04 - 現在ゲノム微生物学会男女共同参画委員
2013/04 - 現在ゲノム微生物学会ニュースレター編集幹事
2013/04 - 現在ゲノム微生物学会男女共同参画委員

Awards (2)：

2014/03/27 - 日本農芸化学会農芸化学奨励賞
2013/03/09 - 日本ゲノム微生物学会日本ゲノム微生物学会研究奨励賞

Association Membership(s) (3)：

日本ゲノム微生物学会 , 環境微生物学会 , 日本農芸化学会

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