2018 - 2022 Comprehensive screening of drug-binding proteins using a detection method for their interactions based on chemical reactions between reactive peptide tags
2015 - 2019 Construction of novel screening method using enzyme reconstitution and creation of bicyclic functional D-peptides
2012 - 2014 Construction of Detection System for Ligand Screening in Solution Using Peptide Generation based on Molecular Interactions
2009 - 2010 Control of amyloidogenic proteins using surface-engineered β-barrel proteins
Hiroaki Hagiwara, Eriho Fujii, Taisei Iwabuchi, Tsuyoshi Takahashi. Development of a β-lactamase activity assay for detecting ligand-protein interactions using an engineered split intein and β-lactamase. Bulletin of the Chemical Society of Japan. 2024. 97. 3
Tsuyoshi Takahashi, Tatsuya Uchibayashi, Nozomi Ishii, Ichiro Matsuo, Yukiko Yoshida, Tadashi Suzuki. Luminescence detection of peptide:N-glycanase activity using engineered split inteins. Chemical communications (Cambridge, England). 2022. 58. 95. 13282-13285
Tsuyoshi Takahashi, Hiroaki Hagiwara. Detecting ligand-protein interactions inside cells using reactive peptide tags and split luciferase. Chemical Communications. 2021. 57. 77. 9906-9909
Misaki Kawase, Meiko Fujioka, Tsuyoshi Takahashi. Activation of Protease and Luciferase Using Engineered Nostoc Punctiforme PCC73102 DnaE Intein with Altered Split Position. ChemBioChem. 2021. 22. 3. 577-584
- 1996 Tokyo Institute of Technology School of Bioscience and Biotechnology
Professional career (1):
Doctor Engineering (Tokyo Institute of Technology)
Work history (2):
Gunma University
Former Institution / Organization Tokyo Institute of Technology Graduate School of Biosciences and Biotechnology Department of Bioengineering Assistant Professor
Awards (2):
2010/11 - 日本ペプチド学会 奨励賞
2001/03 - 株式会社白元 鎌田泉博士論文賞
Association Membership(s) (4):
日本ペプチド学会
, 日本化学会
, Japanese Peptide Society
, The Chemical Sociey of Japan