Rchr
J-GLOBAL ID:200901031542961571
Update date: Feb. 01, 2024
Kusakabe Hirokazu
クサカベ ヒロカズ | Kusakabe Hirokazu
Affiliation and department:
Job title:
Associate Professor
Research field (3):
Environmental effects of chemicals
, Environmental effects of radiation
, Biological resource conservation
Research keywords (8):
精子
, 保存
, 配偶子
, ゲノム
, spermatozoa
, preservation
, Gamete
, Genome
Research theme for competitive and other funds (7):
- 2020 - 2023 Freeze-dying of mammalian oocytes
- 2013 - 2016 哺乳類のフリーズドライ精子における高温耐性獲得に関する研究
- 2004 - 2007 Preservation of mammalian spermatozoa at room temperature
- 2004 - 2006 マウス精子および体細胞の室温保存法に関する基礎的な研究
- 2001 - 2004 Changeable aspects of radiation-induced chromosome aberrations during early development of the zygote
- 2004 - Study on reproductive technology to maintain the genetic integrity of freeze-dried spermatozoa and somatic cells in mammalian species
- 2002 - 2003 Genotoxicity test of food additives
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Papers (35):
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Toshiaki Hino, Hirokazu Kusakabe. A reliable technique for karyotyping mouse oocytes prepared by a gradual fixation/air-drying method followed by multicolour FISH. Biology open. 2023. 12. 12
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Hiroyuki Tateno, Miwa Tamura-Nakano, Hirokazu Kusakabe, Noritaka Hirohashi, Natsuko Kawano, Ryuzo Yanagimachi. Sperm acrosome status before and during fertilization in the Chinese hamster ( Cricetulus griseus ), and observation of oviductal vesicles and globules. Molecular Reproduction and Development. 2021. 88. 12. 793-804
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Hirokazu Kusakabe. Production of mouse fetuses using spermatozoa exposed temporarily to high temperature or continuously to room temperature after freeze-drying in Na+-free/K+-rich EGTA buffer. Cryobiology. 2019. 87. 105-109
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Hirokazu Kusakabe, Hiroyuki Tateno. Prevention of high-temperature-induced chromosome damage in mouse spermatozoa freeze-dried using Ca2+ chelator-containing buffer alkalinized with NaOH or KOH. CRYOBIOLOGY. 2017. 79. 71-77
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H. Watanabe, H. Kusakabe, H. Mori, R. Yanagimachi, H. Tateno. Production of offspring after sperm chromosome screening: an experiment using the mouse model. HUMAN REPRODUCTION. 2013. 28. 2. 531-537
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MISC (11):
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T. Hino, H. Kusakabe, H. Tateno. FOR HOW LONG DOES THE SECOND POLAR BODY OF THE MOUSE-FERTILIZED EGG RETAIN GENETIC STABILITY?. FERTILITY AND STERILITY. 2012. 98. 3. S163-S163
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TATENO Hiroyuki, KUSAKABE Hirokazu, KAMIGUCHI Yujiroh. Occurrence of structural chromosome aberrations, aneuploidy and mosaicism in mouse early cleavage embryos derived from spermatozoa with γ-ray-induced DNA damage. 2011. 28. 2. S112
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H. Watanabe, H. Tateno, H. Kusakabe, Y. Kamiguchi, Y. Fujise, H. Ishikawa, S. Ohsumi, Y. Fukui. Fertilizability and chromosomal integrity of frozen-thawed bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes. REPRODUCTION FERTILITY AND DEVELOPMENT. 2007. 19. 1. 306-306
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KUSAKABE Hirokazu, KAMIGUCHI Yujiroh. O-16. Novel method to improve chromosomal integrity of freeze-dried mouse spermatozoa(Abstracts of the oral and poster presentations) :. Chromosome science. 2006. 9. 4. 119-119
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JL Liu, H Kusakabe, CC Chang, H Suzuki, DW Schmidt, M Julian, R Pfeffer, CL Bormann, XC Tian, R Yanagimachi, et al. Freeze-dried sperm fertilization leads to full-term development in rabbits. BIOLOGY OF REPRODUCTION. 2004. 70. 6. 1776-1781
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Patents (1):
Lectures and oral presentations (33):
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室温・高温保存したフリーズドライ精子における染色体異常誘発の抑制
(日本卵子学会 2016)
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フリーズドライ精子における染色体異常生成とその抑制
(第58回日本生殖医学会 2013)
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凍結乾燥精子の高温耐性獲得と染色体異常の誘発抑制
(染色体学会第62回大会 2011)
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高温(50°C)で保存した凍結乾燥精子におけるDNA損傷と染色分体交換の高頻度誘発
(日本環境変異原学会第回37回大会 2009)
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高温保存した凍結乾燥精子の単一細胞ゲル電気泳動法(コメットアッセイ)によるDNA損傷の検出
(第51回日本不妊学会北海道地方部会・学術講演会 2009)
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Education (2):
- 1990 - 1992 弘前大学大学院 理学研究科 生物学専攻
- 1986 - 1990 Hirosaki University
Professional career (2):
- Doctor(Health Science) (Kyorin University)
- Master(science) (Hirosaki University)
Work history (8):
- 2012/04 - 現在 Asahikawa Medical University School of Medicine
- 2008/07 - 2012/03 Asahikawa Medical University School of Medicine
- 2007/04 - 2008/06 Asahikawa Medical University School of Medicine
- 2002/04 - 2007/03 Asahikawa Medical University School of Medicine
- 1999/10 - 2001/10 Institute for Biogenesis Research Asistant Reseracher
- 1999 - 2001 Assistant Researcher, Institute for Biogenesis
- 1992/04 - 1999/09 (財)食品薬品安全センター秦野研究所 研究技術員
- 1992 - 1999 Researcher, Food and Drug Safety Center/Hatano
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Awards (1):
- 2009/02 - 第51回日本不妊学会北海道地方部会 最優秀演題賞 高温保存した凍結乾燥精子の単一細胞ゲル電気泳動法(コメットアッセイ)によるDNA損傷の検出
Association Membership(s) (5):
JAPAN SOCIETY FOR REPRODUCTIVE MEDICINE
, 日本環境変異原ゲノム学会
, 染色体学会
, The Japanese Environmental Mutagen Society
, The Society of Chromosome Research
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