Fujita Toshitsugu

フジタトシツグ | Fujita Toshitsugu

Affiliation and department：
Job title： Graduate student(doctorate program)

Research field (3)： Applied biochemistry , Molecular biology , Genomics

Research keywords (2)：分子生物学 , Cell Biology

Research theme for competitive and other funds (8)：

2021 - 2024 メチル化CpG結合蛋白質による等温DNA増幅反応阻害機構の解析およびその応用
2018 - 2020 in vitro enChIP法を利用した細胞系列特異的な染色体三次元構造の解明
2015 - 2018 Identification and characterization of interactions between genomic regions using the locus-specific chromatin immunoprecipitation technology
2015 - 2017 遺伝子座特異的クロマチン免疫沈降法によるゲノム機能発現機構の解明
2015 - 2017 in vitro enChIP法の技術基盤の確立およびゲノム機能解析への応用

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Papers (61)：

Mina Ishidoya, Toshitsugu Fujita, Sadatomo Tasaka, Hodaka Fujii. Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status. Analytica chimica acta. 2024. 1302. 342486-342486
Toshitsugu Fujita, Hodaka Fujii. iChIP-SILAC analysis identifies epigenetic regulators of CpG methylation of the p16INK4A gene. FEBS letters. 2024
Toshitsugu Fujita, Shoko Nagata, Hodaka Fujii. Oligoribonucleotide-Mediated Blockade of DNA Extension by Taq DNA Polymerases Increases Specificity and Sensitivity for Detecting Single-Nucleotide Differences. Analytical chemistry. 2023
Minako Yokoyama, Toshitsugu Fujita, Yuka Kadonosawa, Yota Tatara, Daisuke Motooka, Masahito Ikawa, Hodaka Fujii, Yoshihito Yokoayama. Development of transgenic mice overexpressing mouse carbonyl reductase 1. Molecular biology reports. 2023. 50. 1. 531-540
Sachiko Imanishi, Shoko Nagata, Toshitsugu Fujita, Hodaka Fujii. Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma. International journal of experimental pathology. 2022. 103. 6. 245-251

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MISC (5)：

Yuhki Yokoyama, Takashi Takeda, Toshitsugu Fujita, Daisuke Okuzaki, Mami Sugiyama, Kaho Asai, Hidekazu Takahashi, Hodaka Fujii, Masaki Mori, Hirofumi Yamamoto. The regulation mechanism of KLF5 gene expression via three-dimensional genome structure in colorectal cancer. CANCER SCIENCE. 2021. 112. 338-338
清水武史, 馬場啓介, 藤田敏次, 藤井穂高. Detection of single-nucleotide mutations of cancer driver genes and polymorphisms in tissue specimens by ORNi-PCR. 日本癌学会学術総会抄録集(Web). 2020. 79th
武田和, 横山雄起, 藤田敏次, 北川公望, 廣瀬遥香, 高橋秀和, 植村守, 松田宙, 水島恒和, 森正樹, et al. 大腸癌細胞株におけるKLF5エンハンサー領域の同定(Identification of the KLF5 enhancer region in colon cancer cell lines). 日本癌学会総会記事. 2019. 78回. P-3056
馬場啓介, 馬場啓介, 藤田敏次, 田坂定智, 藤井穂高. ORNi-PCR法による肺癌EGFR遺伝子L858R変異とT790M変異の同時検出. 日本分子生物学会年会プログラム・要旨集(Web). 2019. 42nd
Identification of molecules associated with specific genomic regions and analysis of higher-order chromosome conformation using the locus-specific chromatin immunoprecipitation technology. 2017. 68. 3. 189-193

Patents (6)：

Method for detecting target nucleic acid, method for detecting molecule having nucleic acid binding ability, and method for evaluating nucleic acid binding ability
標的核酸領域内の基準配列に対する差異を検出する方法
核酸増幅の特異的抑制方法
内在性DNA配列特異的結合分子を用いる特定ゲノム領域の単離方法
Method for isolating specific genomic regions

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Books (6)：

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Education (3)：

2001 - 2004 Osaka University Graduate School of Science Department of Biological Sciences
1999 - 2001 Shizuoka University Graduate School of Agriculture
1995 - 1999 Shizuoka University Faculty of Agriculture Department of Applied Biological Chemistry

Professional career (1)：

Work history (4)：

Awards (4)：

Association Membership(s) (4)：

日本分子生物学会 , 日本癌学会 , 日本エピジェネティクス研究会 , THE JAPANESE SOCIETY FOR IMMUNOLOGY

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