Research keywords (8)：
Chemical Biology
, Respiratory complex I
, Ubiquinone
, Photoaffinity labeling
, Organic Synthesis
, Respiratory chain
, Bioorganic chemistry
, Biological organic chemistry
Research theme for competitive and other funds (15)：
2022 - 2025 Screening of the proteins involved in uptake of ubiquinone in Saccharomyces cerevisiae using a synthetic ubiquinone probe I
2017 - 2021 Novel pathway of coenzyme Q biosynthesis
2018 - 2021 Inhibition mechanism of epoxycyclohexenediones on the mitochondrial ADP/ATP carrier
2018 - 2019 Generation of “Light-driven” respiratory complex I through chemical modification
2016 - 2018 Conversion of mitochondrial respiratory complex I to a light-driven proton pump by chemical modification
2015 - 2018 Functional analysis of mitochondrial VDAC using synthetic ubiquinone probes
2014 - 2017 Site-specific modification of respiratory complex I for single molecule analysis
2013 - 2015 Molecular mechanism of mitochondrial proteins using ubiquinone as a cofactor
2011 - 2014 Study on the function of ND1 subunit, a key subunit of respiratory complex I
2011 - 2013 Probing the intercellular traffic mechanism of ubiquinone using synthetic molecular probes
2010 - 2011 Probing the structure and function of mitochondrial NADH-ubiquinone oxidoreductase (Complex I) by photoaffinity labeling technique
2009 - 2011 Exploring the reaction mechanism of mitochondrial NADH-ubiquinone oxidoreductase (compelx I) using a specific inhibitor as a molecular probe
2007 - 2009 Synthesis of functional ubiquinone probes; toward characterization of the target proteins
2006 - Synthesis of functional ubiquione probes and analysis of ubiquinone-binding ptotein
2004 - Design synthesis of biologically active compounds acting at respiratory enzymes
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Papers (78)：
Ryo Ikunishi, Ryohei Otani, Takahiro Masuya, Kyoko Shinzawa-Itoh, Tomoo Shiba, Masatoshi Murai, Hideto Miyoshi. Respiratory complex I in mitochondrial membrane catalyzes oversized ubiquinones. Journal of Biological Chemistry. 2023. 105001-105001
Shinpei Uno, Takahiro Masuya, Oleksii Zdorevskyi, Ryo Ikunishi, Kyoko Shinzawa-Itoh, Jonathan Lasham, Vivek Sharma, Masatoshi Murai, Hideto Miyoshi. Diverse reaction behaviors of artificial ubiquinones in mitochondrial respiratory complex I. Journal of Biological Chemistry. 2022. 298. 102075-102075
Daniel N. Grba, James N. Blaza, Hannah R. Bridges, Ahmed-Noor A. Agip, Zhan Yin, Masatoshi Murai, Hideto Miyoshi, Judy Hirst. Cryo-electron microscopy reveals how acetogenins inhibit mitochondrial respiratory complex I. Journal of Biological Chemistry. 2022. 298. 3. 101602-101602
Elcidating the action mechanism of inhibitors against Na+-pumping NADH-quinone oxidoreductase from Vibrio cholera
(Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting 2024)
Comprehensive screening of the proteins involved in the trafficking of coenzyme Q in Saccharomyces cerevisiae using a synthetic ubiquinone probe
(Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting 2024)
Defining the action mechanism of diariyisooxazole-3-caboxamides, small molecule inhibitors targeting the mitochondrial permeability transition
(Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting 2024)
Characterization of the quinone-binding pocket of respiratory complex I using a PC-quinazoline hybrid inhibitor
(Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting 2024)
Characterization of the conformational change of Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae through specific chemical modification
(Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting 2024)
2004 - 2009 Kyoto University Graduate School of Agriculture Division of Applied Life Sciences
2000 - 2004 Kyoto University Faculty of Agriculture
Professional career (1)：
Ph. D in Agriculture (Kyoto University)
Work history (3)：
2018/08 - 現在 Kyoto University Graduate School of Agriculture Associate Professor
2009/04 - 2018/07 Kyoto University Graduate School of Agriculture Assistant Professor
2007/04 - 2009/03 Japan Society for the Promotion of Science JSPS research fellow (DC2)
Committee career (6)：
2023/04 - 現在 Japan Society for Bioscience, Biotechnology, and Agrochemistry Editorial Board Member (Japanese Edition)
2021/04 - 現在 Pesticide Science Society of Japan Committee Member
2018/04 - 現在 日本農薬学会 農薬バイオサイエンス委員会(学術小集会)委員
2021/04 - 2022/03 Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) JSBBA 2022 Annual Meeting Organizing Commitee
2019/04 - 2021/03 Pesticide Science Society of Japan Editorial Board Member (Managing Editor)
2019/04 - 2021/03 Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) JSBBA, Kansai Branch, Committee Member
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Awards (1)：
2019/03 - Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) JSBBA Award for Young Scientists Exploring the structure and function of mitochondrial respiratory complex I through chemical biology approaches
Association Membership(s) (3)：
Japan Bioenergetics Group
, Pesticide Science Society of Japan
, Japan Society for Bioscience, Biotechnology, and Agrochemistry
