Rchr

J-GLOBAL ID：200901079684463091 Update date: Nov. 05, 2024

Honda Yoichi

ホンダヨイチ | Honda Yoichi

Affiliation and department：
Job title： Associate Professor,Associate Professor
Homepage URL (1)： http://www.biomass.kais.kyoto-u.ac.jp/

Research field (5)： Applied microbiology , Landscape science , Environmental agriculture , Wood science , Molecular biology

Research keywords (9)： transformation , genetic engineering , basidomycetes , 環境農学 , 分子遺伝学 , 応用微生物学 , バイオマス変換学 , 分子生物学 , Molecular Biology

Research theme for competitive and other funds (31)：

2022 - 2028 Genetic pollution caused by edible mushrooms cultivation in east Asia and its technical solution
2022 - 2026 Isolation of a safe and eco-friendly cultivar in Lentinula edodes by developing new genome editing technologies
2021 - 2026 Mycelial factory for production of biopharmaceuticals using synthetic biology
2020 - 2023 Bilateral approach using post-genomics and genome editing to elucidate wood decomposition system
2018 - 2022 Surveys and mechanism elucidation of genetic pollution caused by edible mushrooms cultivation in east Asia

2018 - 2022 Modification of the protein secretory system in wood rot fungi

2019 - 2021 Biopharmaceuticals production by combining wood-rot fungi and enzymatic transglycosylation

2019 - 2021 Phase specific omics on wood wafers and genome editing elucidate mechanism for white rot

2015 - 2018 Platform formation for pharmaceutical glycoprotein production system in wood rot fungus

2013 - 2015 Challenge for production of therapic glyco-peptides using gene expression system in wood rot fungi

2009 - 2012 Characterization of unique properties in expression of ligninolytic enzymes by white-rot fungi

2008 - 2010 Evaluation of diversity of wood-deteriorating organisms in tropical plantation forests in search for sustainable management

2007 - 2009 Analytical studies on functions and biosynthesis of metabolism-controlling hydrophobic factors produced by white rot fungus

2004 - 2007 Bio-processing of preservative-treated timbers with biodegrading agents and new energy oplions

2004 - 2007 Molecular breeding of white rot basidiomycete for efficient saccharification in biomass conversion process

2004 - 2005 Treatment of the fermentation inhibitors from wood by Trametes versicolor RC3 Laccase

2002 - 2003 遺伝子組換え担子菌を用いた内分泌攪乱物質分解システムの構築

2001 - 2002 染色体工学を用いた選択的リグニン分解菌の分子育種

2001 - 2001 リグニン分解系を強化した組換え担子菌による内分泌撹乱物質の分解

2000 - 2001 Analysis op-oxidation mchanism of white rot fungi that promate lignin degradation by addition of polyisoprene rubber ; Application to resurce conversion and bioremediation

1999 - 2000 選択的リグニン分解菌の遺伝子工学的解析

1996 - 1997 Studies on ligninolytic activities of ruminant microbes

1996 - 1996 人工的にデザインされたDNA複製オリジンを用いた糸状菌レプリコンの設計

1995 - 1995 担子菌のリグニン分解酵素系の分子生物学的解析と自律複製型ベクターの開発

1994 - 1994 担子菌類における自律複製型ベクター系の開発-広宿主域プラスミドの宿主非依有性ブライミングシステムの応用

森林生物の遺伝的多樣性の解析

木材腐朽菌における遺伝子発現系の開発

担子菌のリグニン分解系の分子生物学的解析

Molecules genetical analysis of biodiversity in forestry organisms

Development of host-vector systems in wood-rot fungi

Molecular biological analysis of lignin bio-degradation by basidiomycetes

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Papers (193)：

Hayase Kojima, Moriyuki Kawauchi, Yuitsu Otsuka, Kim Schiphof, Kenya Tsuji, Akira Yoshimi, Chihiro Tanaka, Shigekazu Yano, Takehito Nakazawa, Yoichi Honda. Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus. Fungal genetics and biology : FG & B. 2024. 175. 103936-103936
Kim Schiphof, Moriyuki Kawauchi, Kenya Tsuji, Akira Yoshimi, Chihiro Tanaka, Takehito Nakazawa, Yoichi Honda. Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus. Fungal Genetics and Biology. 2024. 172. 103893-103893
Takeshi Kobukata, Takehito Nakazawa, Fuga Yamasaki, Junko Sugano, Minji Oh, Moriyuki Kawauchi, Masahiro Sakamoto, Yoichi Honda. Identification of two genes essential for basidiospore formation during the postmeiotic stages in Pleurotus ostreatus. Fungal genetics and biology : FG & B. 2024. 103890-103890
Takehito Nakazawa, Moriyuki Kawauchi, Yuitsu Otsuka, Junxian Han, Daishiro Koshi, Kim Schiphof, Lucía Ramírez, Antonio G Pisabarro, Yoichi Honda. Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences. Applied microbiology and biotechnology. 2024. 108. 1. 217-217
Takuma Narutaki, Ayane Kamiya, Yuma Tsujimoto, Masataka Naruse, Shota Nishida, Moriyuki Kawauchi, Yoichi Honda, Hisatoshi Kamitsuji, Toshitsugu Sato, Takuya Sumita, et al. Marker recycling in Lentinula edodes via 5-fluoroorotic acid counter-selection. FEMS Microbiology Letters. 2024

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MISC (439)：

大塚唯, 河内護之, 辻健也, 吉見啓, 吉見啓, 田中千尋, 田中千尋, 矢野成和, 入江俊一, 中沢威人, et al. Development of a high-density liquid culture strain of the white-rot fungus Pleurotus ostreatus. 日本木材学会大会研究発表要旨集(完全版)(CD-ROM). 2024. 74th
小島隼星, KIM Schiphof, 河内護之, 辻健也, 田中千尋, 田中千尋, JEONG Yong-Hyeon, SHIN Hyun-Jae, 中沢威人, 本田与一. Developing a protocol for mycelial material production using the white-rot fungus Pleurotus ostreatus. 日本木材学会大会研究発表要旨集(完全版)(CD-ROM). 2024. 74th
HAN Junxian, 河内護之, 辻健也, 寺内裕貴, 吉見啓, 吉見啓, 田中千尋, 田中千尋, 中沢威人, 本田与一. Functional analysis of highly expressed hydrophobin hydph16 during wood degradation in the white-rot fungus Pleurotus ostreatus. 日本木材学会大会研究発表要旨集(完全版)(CD-ROM). 2024. 74th
和泉貴哉, 小島隼星, 大塚唯, 河内護之, 辻健也, 吉見啓, 田中千尋, 田中千尋, 矢野成和, 中沢威人, et al. Functional analysis of the transcription factor Swi6, which contributes to the regulation of cell wall synthesis in the white-rot fungus, Pleurotus ostreatus. 日本木材学会大会研究発表要旨集(完全版)(CD-ROM). 2024. 74th
河内護之, 名和義順, 大塚唯, KIM Schiphof, WAN Y., 辻健也, 吉見啓, 吉見啓, 田中千尋, 田中千尋, et al. Analysis of physiological functions of the β-glucan synthase gene of Pleurotus ostreatus for elucidation of cell wall structure of white-rot fungi. 日本木材学会大会研究発表要旨集(完全版)(CD-ROM). 2024. 74th

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Patents (5)：

真正担子菌のリグニン分解能を増加させる方法
薬剤耐性遺伝子の利用
リグノセルロース分解作用を有する白色腐朽菌及びその利用
新規薬剤耐性遺伝子
特許薬剤耐性遺伝子

Books (16)：

Biological activities of mushrooms and their application development
CMC Publisher 2021
機能性糖質素材の開発と食品への応用II (普及版)
2020
きのこの生理機能と応用開発の展望
S&T出版 2017
機能性糖質素材の開発と食品への応用II
シーエムシー出版 2014
講座生存基盤論6 持続型生存基盤論ハンドブック
京都大学学術出版会 2012

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Lectures and oral presentations (85)：

Molecular toolbox for a model white-rotmushroom, Pleurotus ostreatus
(韓国国立園芸薬草科学研究所 2024)
Genome engineering tool box for a model white-rot mushroom, Pleurotus ostreatus
(International workshop by the endowed reserach laboratory 2024)
Genetic modification of white rot fungi, Pleurotus ostreatus
(ジョージア農業大学 2024)
Biotechnology in mushrooms for sustainable future - an invitation to study in Japan -
(ダラット大学 2023)
Biotechnology in mushrooms for sustainable future - an invitation to study in Japan -
(ノンラム大学 2023)

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Works (3)：

担子菌類における高効率な形質転換系の開発ホクト
2000 -
ヒラタケ細胞を使った組換えマンガンペルオキシダーゼの大量生産に関する研究豊田中央研究所
2000 -
担子菌類の形質転換に関する研究タカラアグリ
2000 -

Education (2)：

- 1992 Kyoto University
- 1987 Kyoto University Faculty of Agriculture

Professional career (1)：

Doctor of Agricultural Science (Kyoto University)

Work history (14)：

2022/04 - 現在京都大学大学院農学研究科森林科学専攻教授
2022/01 - 2023/12 OECD 経済開発協力機構 CRP fellow
2022/10 - 2023/03 The University of Shiga Prefecture
2019/08 - 2022/07 Jilin Agricultural University Guest Professor
2021/11 - 2022/01 Kitami Institute of Technology Guest Lecturer

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Committee career (31)：

2016/01/01 - 2019/05/25 Mycological Society Japan -
2019/04 - 日本木材学会編集委員
2019/01 - Mycobiology editor
2019/01/01 - Korean Society of Mycology -
2019 - 日本学術振興会科学研究費委員会委員

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Awards (21)：

2024/03 - 日本木材学会優秀学生発表賞白色腐朽菌ヒラタケを用いた菌糸体マテリアル作成法の検討(指導学生の受賞)
2024/03 - 日本木材学会ポスター賞白色腐朽菌ヒラタケの細胞壁合成制御に寄与する転写因子Swi6の機能解析(指導教員、共著者として)
2023/10 - Best psoter prize Transcription factor Mbp1 controls β-glucan synthesis in basidiomycete Pleurotus ostreatus(指導教員、共著者として)
2023/05 - 日本応用糖質学会ポスター賞細胞壁多糖三重染色法の開発により見えてきた木材腐朽菌にユニークな細胞壁構造 (指導教員、共著者として)
2023/03 - poster prize Characterization of cell wall synthesis regulatory transcription factors Mbp1 and Rlm1 in basidiomycete Pleurotus ostreatus

2023/03 - Excellent student award Isolation of genes essential for basidiospore production in Pleurotus ostreatus using transcriptome analysis and CRISPR/Cas9

2023/03 - ECFG16 poster prize Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9

2022/07 - 日本応用糖質科学会東北支部奨励賞(指導学生の受賞)

2019/11 - 10th Meeting of Asia for Mushromm Scinece Best poster award CRISPR/Cas9-driven molecular breeding of sporeless strain in Pleurotus ostreatus

2019/10 - Asia Mycological Congress Best poster prize CRISPR/Cas9-driven simultaneous gene mutations in both nuclei of dikaryotic strain of Pleurotus ostreatus

2018/09 - 日本きのこ学会学生優秀発表賞(指導教員、共著者として) ヒラタケ及びCeriporiopsis subvermisporaにおけるCRISPR/Cas9によるゲノム編集系の確立

2018/09 - 日本きのこ学会ポスター賞ヒラタケにおける分泌型レポーターアッセイシステムの構築

2018/02 - Best poster prize as the first author Functional analysis of gene expression signals in basidiomycetous fungi using transfection, and random or targeted integration on the chromosome.

2018/02 - Best poster prize Cellulolytic enzyme genes are significantly upregulated by most of the mutations that cause defects in the ligninolytic activity in the white-rot fungus Pleurotus ostreatus

2017/07 - 森喜作記念顕彰会森喜作賞食用きのこ類の遺伝子工学を駆使した分子生物学的な研究

2002 - 日本きのこ学会奨励賞遺伝子組換えを用いたきのこの分子育種に関する研究

2002 - prize for encouragement of research from Inoue Foundation

2000 - ホクト研究奨励賞担子菌類における高効率な形質転換系の開発

2000 - prize for encouragement of research from Hokuto foundation

1995 - 井上研究奨励賞広宿主域プラスミドRSF1010のDNA複製開始機構

1995 - prize for encouragement of research from Japan Society of Mushroom Science and Biotechnology

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Association Membership(s) (6)：

日本きのこ学会 , 日本菌学会 , 日本生化学会 , 日本農芸化学会 , 日本木材学会 , 日本分子生物学会

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