Research field (3):
Applied biochemistry
, Functional biochemistry
, Structural biochemistry
Research keywords (3):
酵素科学
, X線結晶構造解析
, 中性子結晶構造解析
Research theme for competitive and other funds (21):
2024 - 2027 Molecular characterisation of the RyDEN-based translation termination complex in the flavivirus RNA dysfunction
2024 - 2027 High-Temperature X-ray Crystallography on the Proteins from Thermophiles, Opening the Way to Protein Science in Extreme Environments
2024 - 2027 量子論的観点に基づく酵素反応におけるプロトン移動の解析
2023 - 2027 Real-time structural dynamics analysis in protein crystal under controlled environmental conditions (temperature and pH).
2023 - 2026 速度論および熱力学的手法を用いた"in crystallo" 酵素反応解析
2022 - 2024 シリアルフェムト秒結晶構造解析に基づく銅含有アミン酸化酵素の触媒機構の解明
2020 - 2023 非凍結結晶を用いた“in crystallo”酵素反応解析
2020 - 2022 シリアルフェムト秒結晶構造解析による銅含有アミン酸化酵素の触媒機構の解明
2019 - 2022 Elucidation of quinone cofactor biogenesis in copper amine oxidase on the basis of proton structures and flexibility analysis
2017 - 2021 In crystallo thermodynamic analysis of the cofactor conformational change of copper amine oxidase
2016 - 2020 Elucidation of enzyme catalytic mechanism regulating transition state by linking conformational change and fluctuations of the active site
2015 - 2019 Concerted proton/electron transfer mechanism in cofactor-dependent enzyme
2014 - 2018 X-ray and neutron crystallographic structure of copper amine oxidase
2012 - 2016 Reaction specificity of pyridoxal enzymes
2011 - 2013 Structural studies for the refined reaction mechanism of copper amine oxidase.
2011 - 2012 ストレプトコッカス難治性感染症に関わるComA の生化学的解析
2011 - 2012 動的構造情報に基づく銅・TPQ含有アミン酸化酵素の反応解析
2009 - 2011 Multidimensional energy analysis of pyridoxal-enzyme and quinoenzyme reactions
2007 - 2009 Kinetic and X-ray crystallographic analysis of quantum mechanical hydrogen tunneling in enzyme-catalyzed reaction
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Papers (43):
Kenji Fukui, Takeshi Murakawa, Nobumasa Hino, Naoyuki Kondo, Takato Yano. ATP binding controls the molecular function of bacterial MutS2 by mediating closure of the dimeric clamp structure. Structure (London, England : 1993). 2025
Takeshi Murakawa, Kazuo Kurihara, Katsuhiro Kusaka, Motoyasu Adachi, Toshihide Okajima. Neutron Crystallography of a Semiquinone Radical Intermediate of Copper Amine Oxidase. J-PARC Annual Report 2023 Volume 2: Materials and Life Science Experimental Facility (MLF Annual Report). 2025. 7-9
Kenji Fukui, Naoyuki Kondo, Takeshi Murakawa, Seiki Baba, Takashi Kumasaka, Takato Yano. dUTP pyrophosphatases from hyperthermophilic eubacterium and archaeon: Structural and functional examinations on the suitability for PCR application. Protein science : a publication of the Protein Society. 2024. 33. 11. e5185
Takeshi Murakawa. Detection of the reaction process of copper amine oxidase. 2024. 75. 3. 252-256
Takeshi Murakawa, Kazuo Kurihara, Mitsuo Shoji, Naomine Yano, Katsuhiro Kusaka, Yoshiaki Kawano, Mamoru Suzuki, Yasuteru Shigeta, Takato Yano, Motoyasu Adachi, et al. Neutron Crystallography of a Semiquinone Radical Intermediate of Copper Amine Oxidase Reveals a Substrate-Assisted Conformational Change of the Peptidyl Quinone Cofactor. ACS Catalysis. 2023. 12403-12413