2017 - 2020 Exploration and functional analysis of a formaldehyde-driven glycine hydroxymethyltransferase
2017 - 2020 複合微生物群集の合理的設計による有機性廃棄物の二次資源化
2015 - 2019 耐熱性酵素を用いたL-システイン製造技術の開発
2014 - 2017 Studies on molecular mechanisms involved in the nicotinamide-cofactor homeostasis in thermophiles and their application to in vitro metabolic engineering
2011 - 2013 Construction of in vitro artificial metabolic pathway using thermo-tolerant enzymes
2010 - 2012 Development of basic technology to simplify biocatalytic processes for the production of chemicals
2008 - 2010 Genome-wide analysis and its application in Chinese hamster ovary cells
2006 - 2008 Algorithm analysis of chemosensory transduction that controls the behavioral state of living organisms and its application to engineering system design
2006 - 2008 Intracellular kinetic analysis of oxygenases in whole-cell catalysts
2005 - 2007 Construction of high-expression vector had on gene-amplified chromosomal location and its application to human cell
Tomoki Tanaka, Ryoga Sugiyama, Yu Sato, Manami Kawaguchi, Kohsuke Honda, Hiroaki Iwaki, Kenji Okano. Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium. Frontiers in Microbiology. 2024. 15
Yu Sato, Kenji Okano, Kohsuke Honda. Effects of small heat shock proteins from thermotolerant bacteria on the stress resistance of Escherichia coli to temperature, pH, and hyperosmolarity. Extremophiles. 2024. 28. 1
Shunsuke Kato, Koki Takeuchi, Motonao Iwaki, Kentaro Miyazaki, Kohsuke Honda, Takashi Hayashi. Chitin- and Streptavidin-Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery. Angewandte Chemie International Edition. 2023
Tatsuya Hizume, Yu Sato, Hiroaki Iwaki, Kohsuke Honda, Kenji Okano. Subtractive modification of bacterial consortium using antisense peptide nucleic acids. Frontiers in microbiology. 2023. 14. 1321428-1321428
Gladwin Suryatin Alim, Kenji Okano, Kohsuke Honda. One-Step Preparation of Cell-Free ATP Regeneration Module Based on Non-Oxidative Glycolysis Using Thermophilic Enzymes. ChemBioChem. 2022. 23. 16
Unique Microbial Enzymes and Their Contribution to Expanding the Designability of Synthetic Metabolic Pathways
(2021)
In vitro synthetic metabolic pathways consisting of thermophilic enzymes
(The 3rd Sino-Japan Symposium on Biocatalysis and Biotransformation 2020)
Construction of in vitro metabolic pathways with thermophilic enzymes
(2020)
Cysteine production through an in vitro biocatalytic cascade consisting of thermophilic enzymes
(1st Japan-Germany-Switzerland Workshop for Enzyme Technology and Bioprocess Development 2019)