Rchr
J-GLOBAL ID:200901089463368472
Update date: Sep. 24, 2024
Higuchi Yoshiki
ヒグチ ヨシキ | Higuchi Yoshiki
Affiliation and department:
Job title:
Specially Appointed Professor of University of Hyogo
Homepage URL (1):
http://www.sci.u-hyogo.ac.jp/life/biophys1/index-j.html
Research field (4):
Molecular biology
, Biophysics
, Structural biochemistry
, Biochemistry
Research keywords (5):
hydrogenase
, 構造生物学
, タンパク質結晶学
, Structural Biology
, Protein Crystallography
Research theme for competitive and other funds (35):
- 2019 - 2024 Structural basis of reaction mechanism and proton pathway of [NiFe]-hydrogenase
- 2018 - 2023 high speed and local transfer hydrogen and electrons
- 2017 - 2019 Reaction mechanism of [FeFe] hydrogenases
- 2016 - 2018 Activity inhibition mechanism of [NiFe] hydrogenase by oxidative modification of active site cysteine ligand
- 2012 - 2018 Structural Basis of Biological System in Hydrogen Energy Metabolism
- 2015 - 2017 Development of new protein cages using domain swapping
- 2014 - 2017 Development of supramolecular construction techniques of proteins and their functionalization using domain swapping
- 2013 - 2016 Structural chemistry of O2-tolerant [NiFe]-hydrogenases
- 2012 - 2014 Reaction Mechansim of [NiFe]-hydrogenase by Neutron Structural Chmeistry
- 2010 - 2014 Molecular design of nylon oligomer degradation enzyme and its application to bioproduction
- 2011 - 2013 Research and development of the highly-active cathodic enzyme necessary for the practical use of the bio-fuel cell
- 2010 - 2012 Structural Study of the Mechanism of Hydrogen Activation on Ni-enzymes
- 2010 - 2011 Wntシグナル伝達系因子の離合集散を制御するDIXドメインの構造生物学
- 2010 - 2011 Study of Catalytic Mechanism of Hydrogen Activation by Hydrogenase using Neutron Crystal Structure Analysis
- 2006 - 2010 Organometallic and Cluster Chemistry in M+I40etalloenzymes with Reducing Activities
- 2006 - 2009 Structural analysis of nylon oligomer hydrolase and application to enzymatic synthesis of unnatural amides
- 2004 - 2007 Activation mechanism of the Active Site of [NiFe] hydrogenas
- 2002 - 2004 Structural study of the activation mechanism of dihydrogen at the Ni-Fe active site of [NiFe] hydrogenase
- 2001 - 2001 動的・準動的X線結晶解析法による蛋白質間相互作用の研究
- 2000 - 2001 Structural study of [NiFe]hydrogenase
- 1998 - 2001 REGULATION AND INTRACELLULAR LOCALISATION OF PHOSPHOIN OSITIDE-BINDING PROTEINS.
- 2000 - 2000 動的・準動的結晶解析法による蛋白質間相互作用の研究
- 1999 - 1999 Ni-Fe金属中心の構造化学的研究
- 1998 - 1998 次世代大型放射光SPring-8での超分子タンパク質結晶学研究の確立
- 1998 - 1998 Ni-Fe金属中心の構造化学的研究
- 1997 - 1997 次世代大型放射光SPring-8タンパク質結晶解析ビームラインの性能評価
- 1996 - 1997 Structural Study of Hydrogenase
- 1996 - 1997 Crystallographic Study of Molecular Mechanism of DNA Repair by Photolyase
- 1996 - 1996 テトラサイクリン/プロトンアンチポ-タの結晶化とX線構造解析
- 1996 - 1996 16ヘム・タンパク質の結晶構造化学
- 1996 - 1996 タンパク質の立体構造に基づく超分子形成の分子機構の解明
- 1995 - 1995 ニッケル酵素・ヒドロゲナーゼの構造化学的研究
- 1994 - 1995 New Generation of Protein Crystallography
- 1992 - 1994 A Crystallographic Workbench and Its Use in Structure Analysis of Hydrogenase
- 1987 - 1988 Structure and Function of High Molecular Weight Cytochrome C in Desulfovibrio
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Papers (224):
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Chara Karafoulidi-Retsou, Christian Lorent, Sagie Katz, Yvonne Rippers, Hiroaki Matsuura, Yoshiki Higuchi, Ingo Zebger, Marius Horch. Light-Induced Electron Transfer in a [NiFe] Hydrogenase Opens a Photochemical Shortcut for Catalytic Dihydrogen Cleavage. Angewandte Chemie International Edition. 2024
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Ami Kobayashi, Midori Taketa, Keisei Sowa, Kenji Kano, Yoshiki Higuchi, Hideaki Ogata. Structure and function relationship of formate dehydrogenases: an overview of recent progress. IUCrJ. 2023. 10. 5. 544-554
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Takeshi Hiromoto, Koji Nishikawa, Seiya Inoue, Hideaki Ogata, Yuta Hori, Katsuhiro Kusaka, Yu Hirano, Kazuo Kurihara, Yasuteru Shigeta, Taro Tamada, et al. New insights into the oxidation process from neutron and X-ray crystal structures of an O2-sensitive [NiFe]-hydrogenase. Chemical Science. 2023. 35. 14. 9306-9315
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Seiji Negoro, Naoki Shibata, Dai-ichiro Kato, Yusuke Tanaka, Kengo Yasuhira, Keisuke Nagai, Shohei Oshima, Yoko Furuno, Risa Yokoyama, Kaito Miyazaki, et al. X-ray crystallographic and mutational analysis of the NylC precursor: Catalytic mechanism of autocleavage and substrate hydrolysis of nylon hydrolase. The FEBS Journal. 2023
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Catharina J. Kulka-Peschke, Anne-Christine Schulz, Christian Lorent, Yvonne Rippers, Stefan Wahlefeld, Janina Preissler, Claudia Schulz, Charlotte Wiemann, Cornelius C. M. Bernitzky, Chara Karafoulidi-Retsou, et al. Reversible Glutamate Coordination to High-Valent Nickel Protects the Active Site of a [NiFe] Hydrogenase from Oxygen. Journal of the American Chemical Society. 2022. 144. 37. 17022-17032
more...
MISC (45):
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廣本武史, 西川幸志, 樋口芳樹, 玉田太郎. 中性子結晶構造解析を用いたヒドロゲナーゼ酸化型における特徴的な水素結合の観察. 日本結晶学会年会講演要旨集. 2023. 2023
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根来誠司, 武尾正弘, 柴田直樹, 樋口芳樹, 加藤太一郎, 重田育照. ナイロン分解酵素NylB の構造進化,触媒機構とアミド合成への応用. 月刊バイオインダストリー. 2019. 36. 6
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柴田直樹, 末吉由依, 樋口芳樹, 虎谷哲夫. X 線結晶構造に基づくB12 補酵素がラジカル酵素反応を制御する仕組みの解明. 放射光. 2019. 33. 1. 33-42
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SHOMURA Yasuhito, HIGUCHI Yoshiki. Protective mechanism against O2 by Fe-S clusters in [NiFe]-hydrogenases. Biophysics. 2018. 58. 6
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SHOMURA Yasuhito, HIGUCHI Yoshiki. Crystal structures of the NAD+-reducing soluble [NiFe]-hydrogenase. SPring-8 SACLA Research Frontier 2017. 2018. 2017. 26-27
more...
Books (28):
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Hydrogenomics: The Science of Fully Utilizing Hydrogen
2023
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生命金属ダイナミクス : 生体内における金属の挙動と制御
エヌ・ティー・エス 2021 ISBN:9784860437060
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イメージング時代の構造生命科学 : 細胞の動態、膜のないオルガネラ、分子の構造変化をトランススケールに観る
羊土社 実験医学増刊 2020 ISBN:9784758103855
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進化を続ける構造生物学
化学同人 2014 ISBN:9784759815153
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構造生命科学で何がわかるのか,何ができるのか : 最先端のタンパク質解析技術から構造情報の活用事例,創薬展開まで
羊土社 実験医学増刊 2014 ISBN:9784758103398
more...
Lectures and oral presentations (25):
-
Structural Studies on [NiFe]-hydrogenases-Role of water/proton-network for O2-stability
(3rd International Solar Fuels Conference (ISF-3) and International Conference on Artificial Photosynthesis-2019 (ICARP2019) 2019)
-
Role of water network for O2-stability of [NiFe]-hydrogenases
(12th International Hydrogenase Conference 2019 2019)
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Structural study on [NiFe]-hydrogenases
(I2CNER International Workshop, Catalysis for Future Energy Technologies 2019 2019)
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Aiming Towards Comprehensive Structural Chemistry on [NiFe]-hydrogenases (Molecular Mechanism of O2-tolerance)
(3rd International Symposium and Workshop on Functional Genomic and Structural Biology (FGSB2018) 2018)
-
Crystallographic Studies of [NiFe]-hydrogenases
(8th Asian Biological Inorganic Chemistry Conference (AsBIC8) 2016)
more...
Professional career (1):
- Doctor of Science (Osaka University)
Committee career (5):
- 2012/01 - 現在 The Protein Society Editorial Advisory Board of Protein Science
- 2003 - 現在 The Japanese Biochemical Society Councillor
- 2010/04 - 2023/03 The Crystallographic Society of Japan Councillor
- 1996/04 - 1998/03 The Biophysical society of Japan committee member of the editorial board
- 1994/04 - 1996/03 The Crystallographic Society of Japan committee member of the editorial board
Awards (5):
- 2022/12 - Hyogo Prefecture Hyogo Prefecture Education Achievement Award (Governor Commendation) university education
- 2020/04 - Ministry of Education, Culture, Sports, Science and Technology Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology Structural basis of the mechanism of hydrogen metabolism for hydrogenases
- 2017/11 - Hyogo prefecture Hyogo Preecture Science Award Structural studies on hydrogenases
- 2014/10 - Iue Memorial Foundation 38th Iue Culture Prize X-ray structural study and application for the proteins
- 1999/11 - The Crystallographic Society of Japan 1999 Award of Japan Crystallographic Society X-ray Structure Analysis of Hydrogenase
Association Membership(s) (6):
Protein science Society of Japan
, The Biophysical Society of Japan
, Tha Japanese Society for Synchrotron Radiation Research
, The japanese Biochemical Society
, The Chemical Society Of Japan
, The Crystallographic Society of Japane
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