Art

J-GLOBAL ID：200902101886834716   Reference number：96A0886354

A New Method of Media Selection for Protease Refolding by Application of Immobilized Subtilisin Preparation.

固定化ズブチリシンを応用したプロテアーゼ再生のため媒質選択の新法

Author (5)： NOHARA D (Nagoya City Univ., Nagoya, JPN) ,  SAKAKIBARA H (Nagoya City Univ., Nagoya, JPN) ,  KURIMOTO E (Nagoya City Univ., Nagoya, JPN) ,  HAYASHI T (Nagoya City Univ., Nagoya, JPN) ,  SAKAI T (Nagoya City Univ., Nagoya, JPN)
Material： Journal of Fermentation and Bioengineering  (J Ferment Bioeng)
Volume： 82  Issue： 3  Page： 315-318  Publication year： Sep. 1996 
JST Material Number： G0535B  ISSN： 0922-338X  CODEN： JFBIEX  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： immobilized enzyme ,...
Semi thesaurus term： subtilisin ,...

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JST classification (1)： Applications of enzymes  (EB09100B)

Substance index (2)： Guanidinium chloride ,  Acetic acid potassium

Reference (14)：

• ITAKURA, K. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science. 1977, 198, 1056-1063
• CHENG, Y. Stabilization of a degradable protein by its overexpression in Escherichia coli. Gene. 1981, 14, 121-130
• WILLIAMS, D. Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins. Science. 1982, 215, 687-689
• HLODAN, R. Protein folding and its implications for the production of recombinant proteins. Biotechnol. Genet. Eng. Rev. 1991, 9, 47-88
• SAKAI, T. Thermal deactivation and subsequent reactivation of enzymes in connection with precipitates formation. A survey of correct refolding. Prot. Eng. 1990, 3, 368-369

Terms in the title (5)： 固定化 ,  ズブチリシン ,  応用 ,  プロテアーゼ ,  媒質