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J-GLOBAL ID：200902110899137703   Reference number：01A0458493

Construction of a Chimeric Shuttle Plasmid via a Heterodimer System: Secretion of an scFv Protein from Bacillus brevis Cells Capable of Inhibiting Hemagglutination.

ヘテロダイマー法によるキメラなシャトルプラスミドの構築 ヘマグルチニン阻害活性を有するscFvタンパク質のB.brevisによる分泌生産

Author (7)： SHIROZA T (Nihon Univ. School of Dentistry at Matsudo, Chiba, JPN) ,  SHIBATA Y (Nihon Univ. School of Dentistry at Matsudo, Chiba, JPN) ,  HAYAKAWA M (Nihon Univ. School of Dentistry at Matsudo, Chiba, JPN) ,  SHINOZAKI N (Nihon Univ. School of Dentistry at Matsudo, Chiba, JPN) ,  FUKUSHIMA K (Nihon Univ. School of Dentistry at Matsudo, Chiba, JPN) ,  UDAKA S (Tokyo Univ. Agriculture, Tokyo, JPN) ,  ABIKO Y (Nihon Univ. School of Dentistry at Matsudo, Chiba, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 65  Issue： 2  Page： 389-395  Publication year： Feb. 23, 2001 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： Bacillus brevis ,...
Semi thesaurus term： anticoagulunt ,...

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JST classification (2)： Production and application of antigens,antibodies,complements  (ED04030W) ,  Gene manipulation  (EC02050B)

Reference (17)：

• 1) Shibata, Y., Kurihara, K., Takiguchi, H., and Abiko, Y., Construction of a functional single-chain variable fragment antibody against hemagglutinin from Porphyromonas gingivalis. Infect. Immun., 66, 2207-2212 (1998).
• 2) Udaka, S. and Yamagata, H., High level secretion of heterologous proteins by Bacillus brevis. Methods Enzymol., 217, 23-33 (1993).
• 3) Shiroza, T. and Kuramitsu, H.K., Construction of a model secretion system for oral streptococci. Infect. Immun., 61, 3745-3755 (1993).
• 4) Shiroza, T. and Kuramitsu, H.K., Secretion of heterologous proteins by genetically engineered Streptococcus gordonii. Methods Cell Science, 20, 127-136 (1998).
• 5) Palmer, B.R. and Marinus, M.G., The dam and dcm strains of Escherichia coli-a review. Gene, 143, 1-12 (1994).

Terms in the title (8)： ヘテロダイマー ,  キメラ ,  構築 ,  ヘマグルチニン ,  阻害活性 ,  scFv ,  タンパク質 ,  分泌