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J-GLOBAL ID:200902120490031410   Reference number:00A0327053

Effect of zinc in the glycine electrode buffer on detection of alkaline phosphatase activity after polyacrylamide gel electrophoresis.

ポリアクリルアミドゲル電気泳動後のアルカリ性ホスファターゼ活性検出におけるグリシン泳動緩衝液中の亜鉛の効果
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Volume: 44  Issue:Page: 15-19  Publication year: Mar. 15, 2000 
JST Material Number: G0565A  ISSN: 0031-9082  CODEN: SBBKA4  Document type: Article
Article type: 原著論文  Country of issue: Japan (JPN)  Language: JAPANESE (JA)
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Reference (18):
  • DAVIS, BJ. Disc Electrophoresis-II. Method and application to human serum proteins. Ann NY Acad Sci. 1964, 121, 404-427
  • BODANSKY, O. Mechanism of inhibition of phosphatase activity by glycine. J Biol Chem. 1946, 165, 605-613
  • HOFSTEE, BHJ. Alkaline phosphatase. I. Mechanism of action of Zn, Mg, glycine, versene and hydrogen ions. Arch Biochem Biophys. 1955, 59, 352-365
  • EPSTEIN, E. An indigogenic reaction for alkaline phosphatase in disk electrophoresis. Am J Clin Pathol. 1967, 48, 530-534
  • SMITH, I. Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels. Clin Chim Acta. 1968, 19, 499-505
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