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J-GLOBAL ID：200902123092467437   Reference number：01A0990931

Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli.

大腸菌中で発現したBrevibacterium saccharolyticum C-1012由来L-2,3-ブタンジオール脱水素酵素の精製と性質

Author (7)： TAKUSAGAWA Y (Yamanashi Univ., Yamanashi, JPN) ,  OTAGIRI M (Yamanashi Univ., Yamanashi, JPN) ,  UI S (Yamanashi Univ., Yamanashi, JPN) ,  OHTSUKI T (Yamanashi Univ., Yamanashi, JPN) ,  MIMURA A (Yamanashi Univ., Yamanashi, JPN) ,  OHKUMA M (Inst. Physical and Chemical Res., Saitama, JPN) ,  KUDO T (Inst. Physical and Chemical Res., Saitama, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 65  Issue： 8  Page： 1876-1878  Publication year： Aug. 23, 2001 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： Brevibacterium ,...
Semi thesaurus term： Brevibacterium saccharolyticum

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JST classification (1)： Enzyme in general  (EB09010D)

Substance index (1)： 2,3-Butanediol

Reference (9)：

• 1) Ui, S., Matsuyama, N., Masuda, H., and Muraki, H., Mechanism for the formation of 2,3-butanediol stereoisomers in Klebsiella pneumoniae. J. Ferment. Technol., 62, 551-559 (1984).
• 2) Ui, S., Masuda, T., Masuda, H., and Muraki, H., Mechanism for the formation of 2,3-butanediol stereoisomers in Bacillus polymyxa. J. Ferment. Technol., 64, 481-486 (1986).
• 3) Ui, S., Masuda, H., and Muraki, H., Stereospecific and electrophoretic natures of bacterial 2,3-butanediol dehydrogenases. J. Ferment. Technol., 61, 467-471 (1983).
• 4) Ui, S., Otagiri, M., Mimura, A., Dohmae, N., Takio, K., Ohkuma, M., and Kudo, T., Cloning, expression and nucleotide sequence of the L-2,3-butanediol dehydrogenase gene from Brevibacterium saccharolyticum C-1012. J. Ferment. Bioeng., 86, 290-295 (1998).
• 5) Ui, S., Takusagawa, Y., Ohtsuki, T., Mimura, A., Ohkuma, M., and Kudo, T., Stereochemical applications of the expression of the L-2,3-butanediol dehydrogenase gene in Escherichia coli. Lett. Appl. Microbiol., 32, 93-98 (2001).

Terms in the title (7)： 大腸菌 ,  発現 ,  Brevibacterium ,  ブタンジオール ,  脱水素酵素 ,  精製 ,  性質