Art

J-GLOBAL ID：200902131783528346   Reference number：01A0910458

Purification and Properties of Two Malate Dehydrogenases from Candida sp. N-16 Grown on Methanol.

メタノールに生育したCandida sp.N-16から2種のリンゴ酸脱水素酵素の精製と諸性質

Author (4)： YOSHIKAWA J (Chiba Univ., Chiba, JPN) ,  SEKI K (Chiba Univ., Chiba, JPN) ,  SHINOYAMA H (Chiba Univ., Chiba, JPN) ,  FUJII T (Chiba Univ., Chiba, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 65  Issue： 7  Page： 1659-1662  Publication year： Jul. 23, 2001 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *malate dehydrogenase ,...
Semi thesaurus term： Candida

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JST classification (1)： Enzyme in general  (EB09010D)

Substance index (3)： Methanol ,  Oxalacetic acid ,  Malic acid

Reference (10)：

• 1) Hou, C.T., Methylotroph: Microbiology Biochemistry, and Genetics. CRC Press (1984).
• 2) Menedez, J., Delgado, J., and Gancedo, C., Isolation of the Pichia pastoris PYC1 gene encoding pyruvate carboxylase and identification of a suppressor of the pyc phenotype. Yeast, 14, 647-654 (1998).
• 3) Fujii, T. and Tonomura, K., Oxidation of methanol, formaldehyde and formate by a Candida species. Agric. Biol. Chem., 36, 2297-2306 (1972).
• 4) Ohshima, T. and Sakuraba, H., Purification and characterization of malate dehydrogenase from the phototrophic bacterium, Rhodopseudomonas capsulata Biochim. Biophys. Acta, 869, 171-177 (1986).
• 5) Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).

Terms in the title (6)： メタノール ,  生育 ,  Candida ,  リンゴ酸脱水素酵素 ,  精製 ,  性質