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J-GLOBAL ID：200902147325323429   Reference number：00A0559737

Purification and Characterization of Organic Solvent-Stable Lipase from Organic Solvent-Tolerant Pseudomonas aeruginosa LST-03.

有機溶媒耐性微生物Pseudomonas aeruginosa LST-03株由来の有機溶媒に安定なリパーゼの精製と性質

Author (7)： OGINO H (Osaka Prefecture Univ., Osaka, JPN) ,  NAKAGAWA S (Osaka Prefecture Univ., Osaka, JPN) ,  SHINYA K (Osaka Prefecture Univ., Osaka, JPN) ,  MUTO T (Osaka Prefecture Univ., Osaka, JPN) ,  FUJIMURA N (Osaka Prefecture Univ., Osaka, JPN) ,  YASUDA M (Osaka Prefecture Univ., Osaka, JPN) ,  ISHIKAWA H (Osaka Prefecture Univ., Osaka, JPN)
Material： Journal of Bioscience and Bioengineering  (J Biosci Bioeng)
Volume： 89  Issue： 5  Page： 451-457  Publication year： May. 25, 2000 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *Pseudomonas aeruginosa ,...
Semi thesaurus term： Pseudomonas aeruginosa ,...

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JST classification (1)： Enzyme in general  (EB09010D)

Substance index (10)： Decane ,  Dimethyl sulfoxide ,  Ethylene glycol ,  Octane ,  Heptane ,  Isooctane ,  Cyclohexane ,  Tricaproin ,  Octanoic acid methyl ,  Dimethylformamide

Reference (9)：

• KHMELNITSKY, Y. L. Engineering biocatalytic systems in organic me-dia with low water content. Enzyme Microb. Technol. 1988, 10, 710-724
• OGINO, H. Organic-solvent-tolerant bacterium which secrtes organic-solvent-stable lipolyt-ic enzyme. Appl. Environ. Microbiol. 1994, 60, 3884-3886
• OGINO, H. Growth of organic solvent-tolerant Pseudomonas aerugi-nosa LST-03 in the presence of various solvents and production of lipolytic enzyme in the presence of ceclohexane. Biochem. Eng. J. 1999, 4, 1-6
• KUROOKA, S. A novel and simple colorimetric assay for human serum lipase. J. Biochem. 1977, 81, 361-369
• BRADFORD, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of proteindye binding. Anal. Biochem. 1976, 72, 248-254

Terms in the title (8)： 有機溶媒耐性 ,  微生物 ,  Pseudomonas ,  LST ,  有機溶媒 ,  リパーゼ ,  精製 ,  性質