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J-GLOBAL ID：200902148684456899   Reference number：01A0018796

Efficient Cloning and Engineering of Giant DNAs in a Novel Bacillus subtilis Genome Vector.

新規Bacillus subtilisゲノムベクターでの巨大DNAの効率の高いクローニングと遺伝子工学

Author (5)： ITAYA M (Mitsubishi Kasei Inst. Life Sci., Tokyo) ,  NAGATA T (Mitsubishi Kasei Inst. Life Sci., Tokyo) ,  SHIROISHI T (National Inst. Genetics, Mishima) ,  FUJITA K (Mitsubishi Kasei Inst. Life Sci., Tokyo) ,  TSUGE K (Mitsubishi Kasei Inst. Life Sci., Tokyo)
Material： Journal of Biochemistry  (J Biochem)
Volume： 128  Issue： 5  Page： 869-875  Publication year： Nov. 01, 2000 
JST Material Number： F0286A  ISSN： 0021-924X  CODEN： JOBIAO  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： Bacillus subtilis ,...
Semi thesaurus term： recombination

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JST classification (1)： Gene manipulation  (EC02050B)

Reference (17)：

• MANIATIS, T. Molecular Cloning: a Laboratory Manual. 1982
• BURKE, D. T. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987, 236, 806-812
• SHIZUYA, H. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA. 1992, 89, 8794-8797
• PEIRCE, J. Using bacteriophage P1 system to clone high molecular weight genomic DNA. Methods in Enzymology. 1992, 216, 549-574
• YANG, X. W. Homologous recombination based modification in Escherichia coli and germline transmission in transgenenic mice of a bacterial artificial chromosome. Nat. Biotechnol. 1997, 15, 859-865

Terms in the title (6)： Bacillus ,  ゲノム ,  ベクター ,  DNA ,  クローニング ,  遺伝子工学