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J-GLOBAL ID：200902165060856428   Reference number：98A0695908

Kinetic Characterization of Lysine-Specific Metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus Fruiting Bodies.

Grifola frondosa及びPleurotus ostreatus子実体由来リジン特異的メタロエンドペプチダーゼの酵素反応速度論特性

Author (3)： NONAKA T (Saitama Univ., Saitama) ,  HASHIMOTO Y (Saitama Univ., Saitama) ,  TAKIO K (Inst. Physical and Chemical Res. (RIKEN), Saitama)
Material： Journal of Biochemistry  (J Biochem)
Volume： 124  Issue： 1  Page： 157-162  Publication year： Jul. 1998 
JST Material Number： F0286A  ISSN： 0021-924X  CODEN： JOBIAO  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *metalloproteinase ,...
Semi thesaurus term： Grifola frondosa ,...

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JST classification (1)： Enzyme in general  (EB09010D)

Substance index (1)： 2,4-Dinitrophenyl-L-Ser-L-Thr-L-Ala-L-Thr-L-Lys-L-Leu-L-Ser-L-Trp-OH

Reference (17)：

• 1. Schechter, I. and Berger, A. (1967) On the size of the active site in proteases. 1. Papain. Biochem. Biophys. Res. Commun. 27, 157-162
• 2. Nonaka, T., Ishikawa, H., Tsumuraya, Y., Hashimoto, Y., Dohmae, N., and Takio, K. (1995) Characterization of a thermo-stable lysine-specific metal loendopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa. J. Biochem. 118, 1014-1020
• 3. Dohmae, N., Hayashi, K., Miki, K., Tsumuraya, Y., and Hashimoto, Y. (1995) Purification and characterization of intracellular proteinases in Pleurotus ostreatus fruiting bodies. Biosci. Biotechnol. Biochem. 59, 2074-2080
• 4. Nonaka, T., Dohmae, N., Hashimoto, Y., and Takio, K. (1997) Amino acid sequences of metalloendopeptidases specific for acyl-lysine bonds from Grifola frondosa and Pleurotus ostreatus fruiting bodies. J. Biol. Chem. 272, 30032-30039
• 5. Knight, C. G. (1995) Fluorimetric assays of proteolytic enzymes. Methods Enzymol. 248, 18-34

Terms in the title (6)： Grifola ,  Pleurotus ,  子実体 ,  リジン ,  メタロエンドペプチダーゼ ,  酵素反応速度論