Art

J-GLOBAL ID：200902168142166925   Reference number：00A0854176

Pyrithiamine Resistance Gene(ptrA) of Aspergillus oryzae: Cloning, Characterization and Application as a Dominant Selectable Marker for Transformation.

麹菌アスペルギルス・オリゼー由来ピリチアミン耐性遺伝子(ptrA)のクローニングと諸性質の検討および優性選択マーカーとしての利用

Author (3)： KUBODERA T (Hakutsuru Sake Brewing Co. Ltd., Kobe, JPN) ,  YAMASHITA N (Hakutsuru Sake Brewing Co. Ltd., Kobe, JPN) ,  NISHIMURA A (Hakutsuru Sake Brewing Co. Ltd., Kobe, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 64  Issue： 7  Page： 1416-1421  Publication year： Jul. 23, 2000 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： drug resistance ,...
Semi thesaurus term： *Aspergillus oryzae ,...

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JST classification (2)： Structure and chemistry of genes  (EC02020U) ,  Microorganism physiology in general  (EG03051L)

Substance index (2)： Thiamin ,  Pyrithiamine

Reference (26)：

• 1) Gomi, K., Iimura, Y., and Hara, S., Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene. Agric. Biol. Chem., 51, 2549-2555 (1987).
• 2) Mattern, IE., Unkles, S., Kinghorn, J. R., Pouwels, P. H., and van den Hondel, C. A. M. J. J., Transformation of Aspergillus oryzae using the Aspergillus niger pyrG gene. Mol. Gen. Genet., 210, 460-461 (1987).
• 3) Unkles, S. E., Campbell, E. I., de Ruiter-Jacobs, YMJT., Broekhuijsen, M., Marco, J. A., Carrez, D., Contreras, R., van den Hondel, C. A. M. J. J., and Kinghorn, J. R., The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: a convenient and general selection system for filamentous fungal transformation. Mol. Gen. Genet., 218, 99-104 (1989).
• 4) Yamada, O., Lee, B. R., and Gomi, K., Transformation system for Aspergillus oryzae with double auxotrophic mutantions, niaD and sC. Biosci. Biotechnol. Biochem., 61, 1367-1369 (1997).
• 5) Gomi, K., Kitamoto, K., and Kumagai, C., Transformation of the industrial strain of Aspergillus oryzae with the homologus amdS gene as a dominant selectable marker. J. Ferment. Bioeng., 74, 389-391 (1992).

Terms in the title (9)： 麹菌 ,  アスペルギルス・オリゼー ,  ピリチアミン ,  耐性遺伝子 ,  PTRA ,  クローニング ,  性質 ,  優性 ,  選択マーカー