Art

J-GLOBAL ID：200902168663621742   Reference number：02A0673509

A Highly Sensitive Assay for Proteases Using Staphylococcal Protein A Fused with Enhanced Green Fluorescent Protein.

増強された緑色蛍光蛋白質で融合したStaphylococcal蛋白質Aを用いたプロテアーゼの高感度アッセイ

Author (3)： FUJINO H (Katayama Chemical Ind. Co., Amagasaki, JPN) ,  AOKI T (Health Sci. Univ. Hokkaido, Hokkaido, JPN) ,  WATABE H (Health Sci. Univ. Hokkaido, Hokkaido, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 66  Issue： 7  Page： 1601-1604  Publication year： Jul. 23, 2002 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： bacterial protein ,...
Semi thesaurus term： protein A

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JST classification (1)： Enzyme in general  (EB09010D)

Reference (16)：

• 1) Buroker-Kilgore, M. and Wang, K. K., A Coomassie brilliant blue G-250-based colorimetric assay for measuring activity of calpain and other proteases. Anal. Biochem., 208, 387-392 (1993).
• 2) Bickerstaff, G. F. and Zhou, H., Protease activity and autodigestion (autolysis) assays using Coomassie blue dye binding. Anal. Biochem., 210, 155-158 (1993).
• 3) Bolger, R. and Checovich W., A new protease activity assay using fluorescence polarization. Biotechniques, 17, 585-589 (1994).
• 4) Schade, S. Z., Jolley, M. E., Sarauer, B. J., and Simonson, L. G., BODIPY-alpha-casein, a pH-independent protein substrate for protease assays using fluorescence polarization. Anal. Biochem., 243, 1-7 (1996).
• 5) Twining, S. S., Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. Anal. Biochem., 143, 30-34 (1984).

Terms in the title (7)： 増強 ,  緑色蛍光蛋白質 ,  融合 ,  蛋白質 ,  プロテアーゼ ,  高感度 ,  アッセイ