Art

J-GLOBAL ID：200902200657427684   Reference number：08A0591223

Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae

Saccharomyces cerevisiaeにおける変異化真核生物膜蛋白質のハイスループット発現のための改良型方法

Author (20)： ITO Keisuke (Dep. of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo ...) ,  SUGAWARA Taishi (Dep. of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo ...) ,  SHIROISHI Mitsunori (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  TOKUDA Natsuko (Dep. of Medical Chemistry, Kyoto Univ., Fac. of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501, JPN) ,  KUROKAWA Azusa (Dep. of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo ...) ,  MISAKA Takumi (Dep. of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo ...) ,  MAKYIO Hisayoshi (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  YURUGI-KOBAYASHI Takami (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  SHIMAMURA Tatsuro (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  NOMURA Norimichi (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  NOMURA Norimichi (Dep. of Medical Chemistry, Kyoto Univ., Fac. of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501, JPN) ,  MURATA Takeshi (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  MURATA Takeshi (Dep. of Medical Chemistry, Kyoto Univ., Fac. of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501, JPN) ,  ABE Keiko (Dep. of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo ...) ,  IWATA So (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  IWATA So (Dep. of Medical Chemistry, Kyoto Univ., Fac. of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501, JPN) ,  IWATA So (RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, JPN) ,  IWATA So (Div. of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial Coll., London SW7 2AZ, GBR) ,  KOBAYASHI Takuya (Iwata Human Receptor Crystallography Project, ERATO, Japan Sci. and Technol. Agency, Konoe-cho, Yoshida, Sakyo-ku ...) ,  KOBAYASHI Takuya (Dep. of Medical Chemistry, Kyoto Univ., Fac. of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501, JPN)
Material： Biochemical and Biophysical Research Communications  (BBRC)
Volume： 371  Issue： 4  Page： 841-845  Publication year： Jul. 11, 2008 
JST Material Number： B0118A  ISSN： 0006-291X  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *Saccharomyces cerevisiae ,  Eukaryota ,  *Membrane Proteins ,  homologous recombination ,  fusion protein ,  Green Fluorescent Proteins ,  gene cloning ,  *transformation ,  clone ,  *gene expression ,  Mutation ,  mutant ,  primer (genetics) ,  plasmid ,  nucleotide sequence ,  throughput ,  polymerase chain reaction ,  *microorganism ,  *Yeasts ,  *Ascomycetous yeast ,  organism ,  *molecular genetic phenomenon ,  Proteins
Semi thesaurus term： green fluorescent protein ,  high throughput

JST classification (1)： Gene manipulation  (EC02050B)

Terms in the title (6)： Saccharomyces ,  変異 ,  真核生物 ,  膜蛋白質 ,  ハイスループット ,  発現