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J-GLOBAL ID：200902241027259404   Reference number：06A0934306

Analysis of Human Proteins that Have an Affinity to Heavy Metals by Metal-Chelating Column Chromatography

金属キレートカラムクロマトグラフィによる重金属への親和性を有するヒト蛋白の分析

Author (4)： OTSUKA Fuminori (Teikyo Univ., Kanagawa, JPN) ,  KOMATSU-OKUGAITO Miho (Teikyo Univ., Kanagawa, JPN) ,  KOIZUMI Shinji (National Inst. of Occupational Safety and Health, Kawasaki, JPN) ,  OHSAWA Motoyasu (Teikyo Univ., Kanagawa, JPN)
Material： Industrial Health  (Ind Health)
Volume： 44  Issue： 4  Page： 674-678  Publication year： Oct. 2006 
JST Material Number： G0778A  ISSN： 0019-8366  CODEN： INHEA  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *heavy metal ,...
Semi thesaurus term： SDS-polyacrylamide gel electrophoresis ,...

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JST classification (3)： Biochemical analytical methods  (EA03030G) ,  Heavy-metal research and measurement  (SB02070I) ,  Toxicity of chemical substances in general  (GZ02010C)

Reference (15)：

• 1) Porath J, Carlsson J, Olsson I, Belfrage G (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258, 598-99.
• 2) Porath J (1992) Immobilized metal ion affinity chromatography. Protein Expr Purif 3, 263-81.
• 3) Arnold FH (1991) Metal-affinity separations: a new dimension in protein processing. Biotechnology 9, 151-6.
• 4) Aoki Y, Kunimoto M, Shibata Y, Suzuki KT (1986) Detection of metallothionein on nitrocellulose membrane using Western blotting technique and its application to identification of cadmium-binding proteins. Anal Biochem 157, 117-22.
• 5) Mazen A, Gradwohl G, de Murcia G (1988) Zinc-binding proteins detected by protein blotting. Anal Biochem 172, 39-42.

Terms in the title (7)： 金属キレート ,  カラムクロマトグラフィ ,  重金属 ,  親和性 ,  ヒト ,  蛋白 ,  分析