Art

J-GLOBAL ID：200902251165975353   Reference number：03A0840285

Patch Clamp Analysis of a H⁺ Pump Heterologously Expressed in Giant Yeast Vacuoles

酵母の巨大液胞で異種発現したH⁺ポンプのパッチクランプ解析

Author (3)： NAKANISHI Y (Nagoya Univ., Nagoya) ,  YABE I (Univ. Tokyo, Tokyo) ,  MAESHIMA M (Nagoya Univ., Nagoya)
Material： Journal of Biochemistry  (J Biochem)
Volume： 134  Issue： 4  Page： 615-623  Publication year： Oct. 01, 2003 
JST Material Number： F0286A  ISSN： 0021-924X  CODEN： JOBIAO  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： patch-clamp ,...
Semi thesaurus term： H-PPアーゼ

   To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=03A0840285&from=J-GLOBAL&jstjournalNo=F0286A") }}

JST classification (3)： Transport of cell membrane  (EF04020R) ,  Biochemical analytical methods  (EA03030G) ,  Gene manipulation  (EC02050B)

Reference (34)：

• NEHER, E. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature. 1976, 260, 799-802
• HEDRICH, R. Comparative studies on the electrical properties of the H^+ translocating ATRase and pyrophosphatase of the vacuolarlysosomal compartment. EMBO J. 1989, 8, 2835-2841
• NEHER, E. The patch clamp technique. Sci. Amer. 1992, 266, 44-51
• BERTL, A. Use of Saccharomyces cerevisiae for patch-clamp analysis of heterologous membrane proteins :characterization of Kat1, an inward-rectifying K^+ channel from Arabidopsis thaliana, and comparison with endogenous yeast channels and carriers. Proc. Natl. Acad. Sci. USA. 1995, 92, 2701-2705
• KURODA, T. Patch clamp studies on ion pumps of the cytoplasmic membrane of Escherichia coli. Formation, preparation, and utilization of giant vacuole-like structures consisting of everted cytoplasmic membrane. J. Biol. Chem. 1998, 273, 16897-16904

Terms in the title (6)： 酵母 ,  液胞 ,  異種発現 ,  H+ポンプ ,  パッチクランプ ,  解析