Art

J-GLOBAL ID：200902254287083029   Reference number：08A0289400

Development of a two-dimensional electrophoresis method to study soil bacterial diversity

土壌細菌の多様性を研究するための二次元電気泳動法の開発

Author (4)： ISSHI Takumi (Yokohama National Univ., Yokohama, JPN) ,  NARASIMMALU Rajendran (Yokohama National Univ., Yokohama, JPN) ,  AMEMIYA Takashi (Yokohama National Univ., Yokohama, JPN) ,  ITOH Kiminori (Yokohama National Univ., Yokohama, JPN)
Material： Soil Science and Plant Nutrition  (Soil Science & Plant Nutrition)
Volume： 52  Issue： 5  Page： 601-609  Publication year： Oct. 2006 
JST Material Number： W1879A  ISSN： 0038-0768  Document type： Article
Article type： 原著論文  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Thesaurus term： *soil bacterium ,...
Semi thesaurus term： 16S rDNA ,...

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JST classification (2)： Soil organisms  (FB04050N) ,  Microorganism test methods  (EG02020G)

Reference (24)：

• CULLEN, DW. Simple and rapid method for direct extraction of microbial DNA from soil for PCR. Soil. Biol. Biochem. 1998, 30, 983-993
• DIEZ, B. Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques. Appl. Environ. Microbiol. 2001, 67, 2942-2951
• DON, RH. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucl. Acids Res. 1991, 19, 4008
• DULLAGHAN, EM. Two-dimensional bacterial genome display : a method for the genomic analysis of mycobacteria. Microbiology. 2002, 148, 3111-3117
• GILLETTE, WK. POET : Using proteomics to screen pools of open reading frames for protein expression. Mol. Cell. Proteomics. 2005, 4, 1647-1652

Terms in the title (5)： 土壌細菌 ,  多様性 ,  研究 ,  二次元電気泳動法 ,  開発