Art

J-GLOBAL ID：200902255274433680   Reference number：05A0261788

Improvements in the Cell-Free Production of Functional Antibodies Using Cell Extract from Protease-Deficient Escherichia coli Mutant

プロテアーゼ欠損大腸菌変異株由来の細胞抽出液を用いる機能性抗体の無細胞生産における改良

Author (6)： ALI M (Nagoya Univ., Nagoya, JPN) ,  SUZUKI H (Nagoya Univ., Nagoya, JPN) ,  FUKUBA T (Nagoya Univ., Nagoya, JPN) ,  JIANG X (Nagoya Univ., Nagoya, JPN) ,  NAKANO H (Nagoya Univ., Nagoya, JPN) ,  YAMANE T (Nagoya Univ., Nagoya, JPN)
Material： Journal of Bioscience and Bioengineering  (J Biosci Bioeng)
Volume： 99  Issue： 2  Page： 181-186  Publication year： Feb. 25, 2005 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *antibody formation ,...
Semi thesaurus term： Fab fragment

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JST classification (1)： Production and application of antigens,antibodies,complements  (ED04030W)

Reference (23)：

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• 3. Yin, G. and Swartz, J.: Enhancing multiple disulfide bonded protein folding in a cell-free system. Biotechnol. Bioeng., 86, 188–195 (2004).
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• 5. Rungpragayphan, S., Kawarasaki, Y., Imaeda, T., Kohda, K., Nakano, H., and Yamane, T.: High-throughput, cloning-independent protein library, construction by combining single-molecule DNA amplification with in vitro expression. J. Mol. Biol., 318, 395–405 (2002).

Terms in the title (8)： プロテアーゼ ,  欠損 ,  大腸菌 ,  変異株 ,  細胞抽出液 ,  機能性 ,  抗体 ,  細胞