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J-GLOBAL ID:200902255775113774   Reference number:04A0579811

Development of Polymerase Chain Reaction and Comparison with In situ Hybridization for the Detection of Haemophilus parasuis in Formalin-Fixed, Paraffin-Embedded Tissues

フォルマリン固定・パラフィン包埋組織における開発したPCR法in situハイブリダイゼーション法によるHaemophilus parasuisの検出との比較
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Material:
Volume: 66  Issue:Page: 841-845  Publication year: Jul. 25, 2004 
JST Material Number: F0905A  ISSN: 0916-7250  Document type: Article
Article type: 原著論文  Country of issue: Japan (JPN)  Language: ENGLISH (EN)
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Category name(code) classified by JST.
Swine(=pigs)  ,  Animal infectious diseases caused by bacteria  ,  Microorganism test methods 
Reference (15):
  • 1. Chalkley, R. and Hunter, C. 1975. Histone-histone propinquity by aldehyde fixation of chromatin. Proc. Natl. Acad. Sci. U.S.A. 72: 1304-1308.
  • 2. Cheon, D.-S. and Chae, C. 2000. Comparison of virus isolation, reverse transcription-polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine reproductive and respiratory syndrome virus fron naturally aborted fetuses and stillborn piglets. J. Vet. Diagn. Invest. 12: 582-587.
  • 3. Greer, C. E., Peterson, S. L., Kiviat, N. B. and Manos, M. M. 1991. PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time. Am. J. Clin. Pathol. 95: 117-124.
  • 4. Hopwood, D. 1969. Fixatives and fixation: a review. Histochem. J. 1: 323-360.
  • 5. Imai, K., Mase, M., Yamaguchi, S., Yuasa, N. and Nakamura, K. 1998. Detection of chicken anaemia virus DNA from formalin-fixed tissues by polymerase chain reaction. Res. Vet. Sci. 64: 205-208.
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