Art

J-GLOBAL ID：200902259699169264   Reference number：09A0357545

Development of Systemic in vitro Evolution and Its Application to Generation of Peptide-Aptamer-Based Inhibitors of Cathepsin E

カテプシンEのペプチドアプタマに基づく阻害剤の作成への体系的in vitro進化の開発及びそれの応用

Author (19)： KITAMURA Koichiro (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  YOSHIDA Chuya (Dep. of Functional Materials Sci., Fac. of Engineering, Saitama Univ., 255 Shimo-okubo, Saitama 338-8570, JPN) ,  KINOSHITA Yasunori (Dep. of Functional Materials Sci., Fac. of Engineering, Saitama Univ., 255 Shimo-okubo, Saitama 338-8570, JPN) ,  KADOWAKI Tomoko (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  KADOWAKI Tomoko (Dep. of Pharmacology, Kyushu Univ., Higashi-ku, Fukuoka 812-8582, JPN) ,  TAKAHASHI Yoko (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  TAYAMA Takahiro (Dep. of Functional Materials Sci., Fac. of Engineering, Saitama Univ., 255 Shimo-okubo, Saitama 338-8570, JPN) ,  KAWAKUBO Tomoyo (Dep. of Pharmacology, Kyushu Univ., Higashi-ku, Fukuoka 812-8582, JPN) ,  NAIMUDDIN Mohammed (Janusys Corp., #508, Saitama Industrial Technol. Center, 3-12-18 Kami-Aoki, Kawaguchi, Saitama 333-0844, JPN) ,  SALIMULLAH Md. (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  NEMOTO Naoto (Dep. of Functional Materials Sci., Fac. of Engineering, Saitama Univ., 255 Shimo-okubo, Saitama 338-8570, JPN) ,  NEMOTO Naoto (Janusys Corp., #508, Saitama Industrial Technol. Center, 3-12-18 Kami-Aoki, Kawaguchi, Saitama 333-0844, JPN) ,  HANADA Kazunori (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  HUSIMI Yuzuru (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  HUSIMI Yuzuru (Dep. of Functional Materials Sci., Fac. of Engineering, Saitama Univ., 255 Shimo-okubo, Saitama 338-8570, JPN) ,  YAMAMOTO Kenji (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  YAMAMOTO Kenji (Dep. of Pharmacology, Kyushu Univ., Higashi-ku, Fukuoka 812-8582, JPN) ,  NISHIGAKI Koichi (Rational Evolutionary Design of Advanced Biomolecules, Saitama (REDS), Saitama Small Enterprise Promotion Corp. ...) ,  NISHIGAKI Koichi (Dep. of Functional Materials Sci., Fac. of Engineering, Saitama Univ., 255 Shimo-okubo, Saitama 338-8570, JPN)
Material： Journal of Molecular Biology  (J Mol Biol)
Volume： 387  Issue： 5  Page： 1186-1198  Publication year： Apr. 17, 2009 
JST Material Number： D0124B  ISSN： 0022-2836  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *cathepsin ,  *proteinase inhibitor ,  in vitro experiment ,  molecular evolution ,  peptide bond hydrolase ,  enzyme inhibition ,  peptide ,  *gene library ,  binding site ,  gene manipulation ,  nucleotide sequence ,  oligopeptide
Semi thesaurus term： *cDNA library ,  DNA shuffling ,  Endopeptidases ,  cathepsin D ,  *Cathepsin E ,  Aptamers, Peptide

JST classification (2)： Enzyme in general  (EB09010D) ,  Research methods in genetics  (EC01020N)

Substance index (1)： Pepstatin A

Terms in the title (7)： カテプシンE ,  ペプチドアプタマ ,  阻害剤 ,  体系 ,  進化 ,  開発 ,  応用