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J-GLOBAL ID：200902262243944631   Reference number：05A0321387

Application of Quantitative Real-Time PCR for Monitoring the Process of Enrichment of Clones on In Vitro Protein Selection

In vitro蛋白質選択におけるクローン濃縮過程をモニターするための定量的RT-PCRの適用

Author (4)： HORISAWA Kenichi (Keio Univ., Yokohama) ,  DOI Nobuhide (Keio Univ., Yokohama) ,  TAKASHIMA Hideaki (Keio Univ., Yokohama) ,  YANAGAWA Hiroshi (Keio Univ., Yokohama)
Material： Journal of Biochemistry  (J Biochem)
Volume： 137  Issue： 2  Page： 121-124  Publication year： Feb. 01, 2005 
JST Material Number： F0286A  ISSN： 0021-924X  CODEN： JOBIAO  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： RT-PCR ,...
Semi thesaurus term： mRNAディスプレイ ,...

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JST classification (2)： Research methods in genetics  (EC01020N) ,  Proteins and peptides in general  (EB03010N)

Reference (24)：

• DOI, N. Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries. Comb. Chem. High Throughput Screen. 2001, 4, 497-509
• LIPOVSEK, D. In-vitro protein evolution by ribosome display and mRNA display. J. Immunol. Methods. 2004, 290, 51-67
• KONDO, A. Yeast cell-surface display-applications of molecular display. Appl. Microbiol. Biotechnol. 2004, 64, 28-40
• SMITH, G. P. Filamentous fusion phage : novel expression vectors that display cloned antigens on the virion surface. Science. 1985, 228, 1315-1317
• FUJII, I. Evolving catalytic antibodies in a phage-displayed combinatorial library. Nat. Biotechnol. 1998, 16, 463-467

Terms in the title (6)： 蛋白質 ,  クローン ,  濃縮過程 ,  モニター ,  定量 ,  RT-PCR