Art

J-GLOBAL ID：200902279489365827   Reference number：08A0414667

A protocol for immunoaffinity separation of the accumulated ubiquitin-protein conjugates solubilized with sodium dodecyl sulfate

ドデシル硫酸ナトリウムで可溶化した蓄積ユビキチン-蛋白質結合体を免疫親和性分離するプロトコール

Author (8)： SHIMADA Yohta (Dep. of Biochemistry, Jikei Univ. School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, JPN) ,  FUKUDA Takahiro (Div. of Neuropathology, Dep. of Neuroscience, Res. Center for Medical Sci., Jikei Univ. School of Medicine, 3-25-8 ...) ,  AOKI Katsuhiko (Dep. of Biochemistry, Jikei Univ. School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, JPN) ,  YUKAWA Toyokazu (Dep. of Biochemistry, Jikei Univ. School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, JPN) ,  YUKAWA Toyokazu (Div. of Gastroenterology and Hepatology, Dep. of Internal Medicine, Jikei Univ. School of Medicine, 3-25-8 ...) ,  IWAMURO Shawichi (Dep. of Biology, Fac. of Sci., Toho Univ., 2-2-1 Miyama, Funabashi, Chiba 274-8510, JPN) ,  OHKAWA Kiyoshi (Dep. of Biochemistry, Jikei Univ. School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, JPN) ,  TAKADA Koji (Dep. of Biochemistry, Jikei Univ. School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, JPN)
Material： Analytical Biochemistry  (Anal Biochem)
Volume： 377  Issue： 1  Page： 77-82  Publication year： Jun. 01, 2008 
JST Material Number： H0177B  ISSN： 0003-2697  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *immunoprecipitation ,  *ubiquitin ,  *protein complex ,  solubilizing agent ,  Niemann-Pick disease ,  Mus musculus ,  K562 cell ,  Brain ,  Amylose ,  Proteins ,  thermal stimulation ,  sample preparation ,  aliphatic alcohol ,  inorganic acid ester ,  cycloamylose ,  flotillin-1 ,  protein renaturation ,  polyoxyethylene sorbitan monolaurate ,  *bioassay ,  *immunoassay ,  Myomorpha
Semi thesaurus term： Tween20 ,  Niemann-Pick Disease, Type C

JST classification (2)： Proteins and peptides in general  (EB03010N) ,  Production and application of antigens,antibodies,complements  (ED04030W)

Substance index (1)： Dodecyl sodium sulfate

Terms in the title (8)： ドデシル硫酸ナトリウム ,  可溶化 ,  蓄積 ,  ユビキチン ,  蛋白質 ,  結合体 ,  免疫親和性 ,  プロトコール