Rapid Detection and Differentiation of the Major Mycoplasma Contaminants in Cell Cultures Using Real-Time PCR with SYBR Green I and Melting Curve Analysis

HARASAWA Ryo; MIZUSAWA Hiroshi; FUJII Masashi; YAMAMOTO Junko; MUKAI Hiroyuki; UEMORI Takashi; ASADA Kiyozo; KATO Ikunoshin

Art

J-GLOBAL ID：200902280456614129 Reference number：05A0816120

Rapid Detection and Differentiation of the Major Mycoplasma Contaminants in Cell Cultures Using Real-Time PCR with SYBR Green I and Melting Curve Analysis

SYBRグリーンIを用いたリアルタイムPCR法と融解曲線法による細胞培養中の主要なマイコプラズマ汚染物の迅速な検出及び判別

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Material：
Volume： 49 Issue： 9 Page： 859-863 (J-STAGE) Publication year： 2005
JST Material Number： F0715A ISSN： 0385-5600 Document type： Article
Article type：短報 Country of issue： Japan (JPN) Language： ENGLISH (EN)

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Microorganism test methods , Bioassay , Gene manipulation

Reference (7)：

HARASAWA, R. PCR : application of nested PCR to detection of mycoplasmas. Molecular and diagnostic procedures in mycoplasmology. 1996, 75-79
HARASAWA, R. Sensitive detection of mycoplasmas in cell cultures by using two-step polymerase chain reaction. Rapid diagnosis of mycoplasmas. 1993, 227-232
MCGARRITY, G. J. Mycoplasmas and tissue culture cells. Mycoplasmas, molecular biology and pathogenesis. 1992, 445-454
TANG, J. A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture. J. Microbiol. Methods. 2000, 39, 121-126
UEMORI, T. Amplification of the 16S-23S spacer region in rRNA operons of mycoplasmas. Syst. Appl. Microbiol. 1992, 15, 181-186

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