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J-GLOBAL ID：200902281457874031   Reference number：05A0651709

Rapid Species Identification and Partial Strain Differentiation of Clostridium butyricum by PCR Using 16S-23S rDNA Intergenic Spacer Regions

16S-23S rDNA遺伝子間スペーサー領域を用いたPCR法によるClostridium butyricumの迅速な種同定と一部の株の識別

Author (6)： NAKANISHI Shusuke (Dep. of Molecular Bacteriology, Inst. of Health Biosciences, The Univ. of Tokushima Graduate School) ,  NAKANISHI Shusuke (Miyarisan Pharmaceutical Co. Res. Lab.) ,  KUWAHARA Tomomi (Dep. of Molecular Bacteriology, Inst. of Health Biosciences, The Univ. of Tokushima Graduate School) ,  NAKAYAMA Haruyuki (Dep. of Molecular Bacteriology, Inst. of Health Biosciences, The Univ. of Tokushima Graduate School) ,  TANAKA Mamoru (Miyarisan Pharmaceutical Co. Res. Lab.) ,  OHNISHI Yoshinari (Dep. of Molecular Bacteriology, Inst. of Health Biosciences, The Univ. of Tokushima Graduate School)
Material： Microbiology and Immunology  (Microbiol Immunol)
Volume： 49  Issue： 7  Page： 613-621 (J-STAGE)  Publication year： 2005 
JST Material Number： F0715A  ISSN： 0385-5600  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： polymerase chain reaction ,...
Semi thesaurus term： *Clostridium ,...

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JST classification (1)： Microorganism morphology and taxonomy  (EG03030Y)

Reference (25)：

• AURELI, P. Two cases of type E infant botulism caused by neurotoxigenic Clostridium butyricum in Italy. J. Infect. Dis. 1986, 154, 207-211
• BIDET, P. Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing. FEMS Microbiol. Lett. 1999, 175, 261-266
• BLAIOTTA, G. 16S-23S rDNA intergenic spacer region polymorphism of Lactococcus garvieae, Lactococcus raffinolactis and Lactococcus lactis as revealed by PCR and nucleotide sequence analysis. Syst. Appl. Microbiol. 2002, 25, 520-527
• DOLZANI, L. Typing of Staphylococcus aureus by amplification of the 16S-23S rRNA intergenic spacer sequences. FEMS Microbiol. Lett. 1994, 119, 167-173
• EZAKI, T. Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J. Clin. Microbiol. 1988, 26, 1708-1713

Terms in the title (7)： rDNA ,  スペーサー領域 ,  PCR法 ,  Clostridium ,  種同定 ,  株 ,  識別