Art

J-GLOBAL ID：200902285980079186   Reference number：07A0475352

Polypeptide linkers suitable for the efficient production of dimeric scFv in Escherichia coli

Escherichia coliの二量体状scFvの効率的生産に適したポリペプチドリンカー

Author (4)： KUMADA Yoichi (Dep. of Bioscience and Biotechnology, Okayama Univ., 3-1-1 Tsushimanaka, Okayama 700-8530, JPN) ,  KAWASAKI Tomomi (Graduate School of Sci. and Technol., Kobe Univ., Kobe 657-8501, JPN) ,  KIKUCHI Yasufumi (Graduate School of Sci. and Technol., Kobe Univ., Kobe 657-8501, JPN) ,  KATOH Shigeo (Graduate School of Sci. and Technol., Kobe Univ., Kobe 657-8501, JPN)
Material： Biochemical Engineering Journal  (Biochem Eng J)
Volume： 35  Issue： 2  Page： 158-165  Publication year： Jul. 15, 2007 
JST Material Number： W1014A  ISSN： 1369-703X  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *immunoglobulin fragment ,...
Semi thesaurus term： Escherichia coli ,...

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JST classification (2)： Production and application of antigens,antibodies,complements  (ED04030W) ,  Biological function  (EB03040U)

Substance index (1)： Bisphenol A

Reference (16)：

• A.A. Kortt, R.L. Malby, J.B. Caldwell, L.C. Gruen, N.M. Ivancic, M.C. Lawrence, G.J. Howlett, R.G. Webster, P.J. Hudson, P.M. Calman, Recombinant antineuraminidase single chain Fv antibody: characterization, formation of dimer and higher molecular mass multimers and solution of the crystal structure of the scFv-neuraminidase complex, Eur. J. Biochem. 221 (1994) 151-157.
• A. Plückthun, P. Pack, New protein engineering approaches to multivalent and bi-specific antibody fragments, Immunotechnology 3 (1997) 83- 105.
• A.A. Kortt, M. Lah, G.W. Oddie, L.C. Gruen, J.E. Burn, L.A. Pearce, J.L. Atwell, A.J. McCoy, G.J. Howlett, D.W. Metzger, R.G. Webster, P.J. Hudson, Single chain Fv fragments of anti-neuraminidase antibody NC10 containing five and ten residue linkers form dimers and with zero residue linker a trimer, Protein Eng. 10 (1997) 423-428.
• J. Atwell, K.A. Breheney, L.J. Lawrence, A.J. McCoy, A.A. Kortt, P.J. Hudson, scFv multimers: length of the linker between VH and VL domains dictates precisely the transition between diabodies and tirabodies, Protein Eng. 12 (1999) 597-604.
• G. Coia, P.J. Hudson, G.G. Lilley, Construction of recombinant extended single-chain antibody peptide conjugates for use in the diagnosis of HIV1 and HIV2, J. Immunol. Meth. 192 (1996) 13-23.

Terms in the title (3)： Escherichia ,  二量体 ,  scFv