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J-GLOBAL ID：200902294806538383   Reference number：06A0170721

Recombinant α-Glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, Purification and Characterization

Aspergillus niger由来組換えα-グルコシダーゼのEmericella nidulansでの過剰発現,精製および諸性質

Author (8)： OGAWA Masahiro (Nihon Univ., Fujisawa, JPN) ,  NISHIO Toshiyuki (Nihon Univ., Fujisawa, JPN) ,  MINOURA Kayo (Nihon Univ., Fujisawa, JPN) ,  UOZUMI Takeshi (Meiji Univ., Kawasaki, JPN) ,  WADA Masato (Nihon Univ., Fujisawa, JPN) ,  HASHIMOTO Noriko (Nihon Univ., Fujisawa, JPN) ,  KAWACHI Ryu (Nihon Univ., Fujisawa, JPN) ,  OKU Tadatake (Nihon Univ., Fujisawa, JPN)
Material： Journal of Applied Glycoscience  (応用糖質科学)
Volume： 53  Issue： 1  Page： 13-16  Publication year： Jan. 20, 2006 
JST Material Number： F0614A  ISSN： 1344-7882  CODEN： JAGLFX  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： recombinant DNA ,...
Semi thesaurus term： recombination ,...

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JST classification (2)： Enzyme in general  (EB09010D) ,  Gene expression  (EC02040Q)

Reference (18)：

• 1) W. Hakamata, T. Nishio and T. Oku: Synthesis of p-nitrophenyl 3-and 6-deoxy-α-D-glucopyranosides and their specificity to rice α-glucosidase. J. Appl. Glycosci., 46, 459-463 (1999).
• 2) W. Hakamata, T. Nishio, R. Sato, T. Mochizuki, K. Tsuchiya, M. Yasuda and T. Oku: Synthesis of monomethyl derivatives of p-nitrophenyl α-D-gluco, galacto, and mannopyranosides and their hydrolytic properties against α-glycosidases. J. Carbohydr. Chem., 19, 359-377 (2000).
• 3) T. Nishio, W. Hakamata, A. Kimura, S. Chiba, A. Takatsuki, R. Kawachi and T. Oku: Glycon specificity profiling of α-glucosidases using monodeoxy and mono-O-methyl derivatives of p-nitrophenyl α-D-glucopyranoside. Carbohydr. Res., 337, 629-634 (2002).
• 4) T. Nishio: Effect of sugar hydroxyl groups on activities and specificities of α-glucosidase and lipase. J. Appl. Glycosci., 49, 45-55 (2002).
• 5) T. Nishio: Glycon specificity profiling of α-glycosidases using partially modified substrates. in Recent Development in Carbohydrate Research, S.G. Pandalai, ed., 1st Ed., Transword Research Network Press, Kerala, pp. 67-79 (2003).

Terms in the title (7)： Aspergillus ,  組換え ,  α-グルコシダーゼ ,  Emericella ,  過剰発現 ,  精製 ,  性質