フラボノイド3′,5′-ヒドロキシラ-ゼ活性を有する蛋白質及びそれをコ-ドする核酸

Inventor： , , , ,
Applicant, Patent owner：
Agent (1)：石田敬 (外2名)
Gazette classification：公開公報
Application number (International application number)：1999190943
Publication number (International publication number)：2000023686
Application date： Jul. 08, 1992
Publication date： Jan. 25, 2000
Summary：

【要約】【課題】フラボノイド系色素の合成に関与する酵素をコードする遺伝子の提供。【解決手段】ペチュニア、ベーベナ、ヒエンソウ、ブドウ、アイリス、フリージア、アジサイ、シクラメン、ホテト、パンジー、ナスなどに由来するフラボノイド3′,5′-ヒドロキシラーゼをコードする遺伝子又はその修飾体が提供され、この遺伝子を植物に挿入することにより、例えば、花の色を人工的に変えた植物などを得ることができる。

Claim (excerpt)：

次のアミノ酸配列(III )又は(IV): (III )Met Met Leu Leu Thr Glu Leu Gly Ala Ala Thr Ser Ile Phe Leu Ile 1 5 10 15Ala His Ile Ile Ile Ser Thr Leu Ile Ser Lys Thr Thr Gly Arg His 20 25 30Leu Pro Pro Gly Pro Arg Gly Trp Pro Val Ile Gly Ala Leu Pro Leu 35 40 45Leu Gly Ala Met Pro His Val Ser Leu Ala Lys Met Ala Lys Lys Tyr 50 55 60Gly Ala Ile Met Tyr Leu Lys Val Gly Thr Cys Gly Met Ala Val Ala 65 70 75 80Ser Thr Pro Asp Ala Ala Lys Ala Phe Leu Lys Thr Leu Asp Ile Asn 85 90 95Phe Ser Asn Arg Pro Pro Asn Ala Gly Ala Thr His Leu Ala Tyr Asn 100 105 110Ala Gln Asp Met Val Phe Ala His Tyr Gly Pro Arg Trp Lys Leu Leu 115 120 125Arg Lys Leu Ser Asn Leu His Met Leu Gly Gly Lys Ala Leu Glu Asn 130 135 140Trp Ala Asn Val Arg Ala Asn Glu Leu Gly His Met Leu Lys Ser Met145 150 155 160Ser Asp Met Ser Arg Glu Gly Gln Arg Val Val Val Ala Glu Met Leu 165 170 175Thr Phe Ala Met Ala Asn Met Ile Gly Gln Val Met Leu Ser Lys Arg 180 185 190Val Phe Val Asp Lys Gly Val Glu Val Asn Glu Phe Lys Asp Met Val 195 200 205Val Glu Leu Met Thr Ile Ala Gly Tyr Phe Asn Ile Gly Asp Phe Ile 210 215 220Pro Cys Leu Ala Trp Met Asp Leu Gln Gly Ile Glu Lys Arg Met Lys225 230 235 240Arg Leu His Lys Lys Phe Asp Ala Leu Leu Thr Lys Met Phe Asp Glu 245 250 255His Lys Ala Thr Thr Tyr Glu Arg Lys Gly Lys Pro Asp Phe Leu Asp 260 265 270Val Val Met Glu Asn Gly Asp Asn Ser Glu Gly Glu Arg Leu Ser Thr 275 280 285Thr Asn Ile Lys Ala Leu Leu Leu Asn Leu Phe Thr Ala Gly Thr Asp 290 295 300Thr Ser Ser Ser Ala Ile Glu Trp Ala Leu Ala Glu Met Met Lys Asn305 310 315 320Pro Ala Ile Leu Lys Lys Ala Gln Ala Glu Met Asp Gln Val Ile Gly 325 330 335Arg Asn Arg Arg Leu Leu Glu Ser Asp Ile Pro Asn Leu Pro Tyr Leu 340 345 350Arg Ala Ile Cys Lys Glu Thr Phe Arg Lys His Pro Ser Thr Pro Leu 355 360 365Asn Leu Pro Arg Ile Ser Asn Glu Pro Cys Ile Val Asp Gly Tyr Tyr 370 375 380Ile Pro Lys Asn Thr Arg Leu Ser Val Asn Ile Trp Ala Ile Gly Arg385 390 395 400Asp Pro Gln Val Trp Glu Asn Pro Leu Glu Phe Asn Pro Glu Arg Phe 405 410 415Leu Ser Gly Arg Asn Ser Lys Ile Asp Pro Arg Gly Asn Asp Phe Glu 420 425 430Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Thr Arg Met 435 440 445Gly Ile Val Met Val Glu Tyr Ile Leu Gly Thr Leu Val His Ser Phe 450 455 460Asp Trp Lys Leu Pro Ser Glu Val Ile Glu Leu Asn Met Glu Glu Ala465 470 475 480Phe Gly Leu Ala Leu Gln Lys Ala Val Pro Leu Glu Ala Met Val Thr 485 490 495Pro Arg Leu Gln Leu Asp Val Tyr Val Pro 500 505 (IV)Met Val Leu Leu Ser Glu Leu Ala Ala Ala Thr Leu Ile Phe Leu Thr 1 5 10 15Thr His Ile Phe Ile Ser Thr Leu Leu Ser Ile Thr Asn Gly Arg Arg 20 25 30Leu Pro Pro Gly Pro Arg Gly Trp Pro Val Ile Gly Ala Leu Pro Leu 35 40 45Leu Gly Ala Met Pro His Val Ser Leu Ala Lys Met Ala Lys Lys Tyr 50 55 60Gly Ala Ile Met Tyr Leu Lys Val Gly Thr Cys Gly Met Val Val Ala 65 70 75 80Ser Thr Pro Asp Ala Ala Lys Ala Phe Leu Lys Thr Leu Asp Leu Asn 85 90 95Phe Ser Asn Arg Pro Pro Asn Ala Gly Ala Thr His Leu Ala Tyr Gly 100 105 110Ala Gln Asp Met Val Phe Ala His Tyr Gly Pro Arg Trp Lys Leu Leu 115 120 125Arg Lys Leu Ser Asn Leu His Met Leu Gly Gly Lys Ala Leu Glu Asn 130 135 140Trp Ala Asn Val Arg Ala Asn Glu Leu Gly His Met Leu Lys Ser Met145 150 155 160Phe Asp Met Ser Arg Glu Gly Glu Arg Val Val Val Ala Glu Met Leu 165 170 175Thr Phe Ala Met Ala Asn Met Ile Gly Gln Val Ile Leu Ser Lys Arg 180 185 190Val Phe Val Asn Lys Gly Val Glu Val Asn Glu Phe Lys Asp Met Val 195 200 205Val Glu Leu Met Thr Thr Ala Gly Tyr Phe Asn Ile Gly Asp Phe Ile 210 215 220Pro Cys Leu Ala Trp Met Asp Leu Gln Gly Ile Glu Lys Gly Met Lys225 230 235 240Arg Leu His Lys Lys Phe Asp Ala Leu Leu Thr Lys Met Phe Asp Glu 245 250 255His Lys Ala Thr Ser Tyr Glu Arg Lys Gly Lys Pro Asp Phe Leu Asp 260 265 270Cys Val Met Glu Asn Arg Asp Asn Ser Glu Gly Glu Arg Leu Ser Thr 275 280 285Thr Asn Ile Lys Ala Leu Leu Leu Asn Leu Phe Thr Ala Gly Thr Asp 290 295 300Thr Ser Ser Ser Ala Ile Glu Trp Ala Leu Ala Glu Met Met Lys Asn305 310 315 320Pro Ala Ile Leu Lys Lys Ala Gln Gly Glu Met Asp Gln Val Ile Gly 325 330 335Asn Asn Arg Arg Leu Leu Glu Ser Asp Ile Pro Asn Leu Pro Tyr Leu 340 345 350Arg Ala Ile Cys Lys Glu Thr Phe Arg Lys His Pro Ser Thr Pro Leu 355 360 365Asn Leu Pro Arg Ile Ser Asn Glu Pro Cys Ile Val Asp Gly Tyr Tyr 370 375 380Ile Pro Lys Asn Thr Arg Leu Ser Val Asn Ile Trp Ala Ile Gly Arg385 390 395 400Asp Pro Glu Val Trp Glu Asn Pro Leu Glu Phe Tyr Pro Glu Arg Phe 405 410 415Leu Ser Gly Arg Asn Ser Lys Ile Asp Pro Arg Gly Asn Asp Phe Glu 420 425 430Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Thr Arg Met 435 440 445Gly Ile Val Met Val Glu Tyr Ile Leu Gly Thr Leu Val His Ser Phe 450 455 460Asp Trp Lys Leu Pro Ser Glu Val Ile Glu Leu Asn Met Glu Glu Ala465 470 475 480Phe Gly Leu Ala Leu Gln Lys Ala Val Pro Leu Glu Ala Met Val Thr 485 490 495Pro Arg Leu Pro Ile Asp Val Tyr Ala Pro Leu Ala 500 505に対して60%以上のホモロジーを有するアミノ酸配列をコードするヌクレオチド配列、又はそれに対して相補的なヌクレオチド配列を有する核酸であって、フラボノイド3′,5′-ヒドロキシラーゼ活性を有する蛋白質をコードする核酸。

IPC (4)：

C12N 15/09 ZNA , A01H 5/00 , C12N 5/10 , C12N 9/02

FI (4)：

C12N 15/00 ZNA A , A01H 5/00 A , C12N 9/02 , C12N 5/00 C

Return to Previous Page