J-GLOBAL ID:200904012623726573  Research Project code:0450021064 Update date:Dec. 15, 2003

Development of ischemic model for animal experiment using MRI: Development of ischemic model for animal experiment using MRI

Study period:2002 - 0
Organization (1):
Investigating Researcher (1):
Research overview:
A remote control transient embolic method was developed to prepare a mesocephalic and cerebral artery occlusive stroke model of rat in MRI. External carotid artery of rat was ligated in advance and a nylon thread was advanced via an inserted catheter to just in front of starting point of mesocephalic and cerebral artery and fixed in the bore of MRI. Rat was anesthetized with 0.5-1.0% halothane via trachea inserted catheter and the body temperature was determined with a platinum electrode inserted in the rectum. The body temperature was maintained by circulation of warm water at 37 degree C and blood pressure was determined via femoral artery catheter. Blood oxygen partial pressure and pulse rate were simultaneously determined with a pulse oximeter from a hind palm and made capable to determine arterial blood gas by drawing arterial blood if necessary. In addition, fibers for laser Doppler determination of cerebral blood flow volume were directly adhered on the scalp of both hemispheres with a cyanoacrylate adhesive Aronalpha to monitor the changes of blood flow in actual time and to confirm occlusion and reperfusion. The blood vessel can be occluded by advancing the nylon thread and re-opened by drawing back the thread. Pathological changes of cerebral tissue before occlusion, during occlusion and after occlusion of cerebral artery were monitored with the progress of time by using the model. Determinations of T2 value with proton and ADC value and others with diffused weighted images in three directions were carried out.

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