J-GLOBAL ID:200904030740067120  Research Project code:0450021005 Update date:Dec. 15, 2003

Experimental studies: Effect of basic fibroblast growth factor on experimental focal ischemia

実験的研究 塩基性線維芽細胞増殖因子の脳虚血におよぼす影響
Study period:2002 - 0
Research overview:
Basic fibroblast growth factor (bFGF) has an angioplastic action, thus its application to neovasularization therapy such as cardiac infarction and peripheral artery obstruction is under investigation. While its nerve cell protective action is focused attention. Cerebral protective action of bFGF at the time of ischemia has been reported in many studies, however, no study for the assessment with MRI, particularly long term influence has done. In the present study, influence of bFGF administration (45μg /kg/hr, 3 hr, iv) 30 minutes after incidence of ischemia was assessed with an improved 4.7T MRI (after 1, 7 and 14 days) and a histological method (after 14 days) using permanently obstructed rat mesencephalon and cerebral artery model. MRI (T2WI, DWI) confirmed the reduction of damaged tissue due to ischemia on day 1, 7 and 14 in bFGF group in comparison to those of control group (saline solution), exhibiting protective action of bFGF for ischemic brain. Confirmation of damaged site with histological method and investigation of new blood vessel with an immune-staining method using bromodeoxy-uridine (BrdU) are under study.

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