Proj
J-GLOBAL ID:200904033293908227  Research Project code:0250005672 Update date:Mar. 04, 2002

Efficient preparation method of embryogenic callus in Kentucky blue grass

ケンタッキーブルーグラスにおける効率的なエンブリオジェニックカルス作成法
Study period:1998 - 1998
Research overview:
In order to prepare efficient preparation of embryogenic callus in Kentucky blue grass having a high re-differentiation rate, callus is induced in a dark condition at 20 degree Celsius by adding 2 mg/L of 2, 4-D and 0.5 mg/L of 6BA to the modified MS culture media and subcultured at least within one month. Re-differentiation rate of about 40% is obtained in the variety Merion.
Research program: Ordinary Research
Ministry with control over the research :
Ministry of Agriculture, Forestry and Fisheries

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