J-GLOBAL ID:200904092225739634  Research Project code:0250000031 Update date:Sep. 29, 2004

The basic research on advanced utilization of Aspergillus oryzae as protein production cell factory: Research on the regulation system of protein production by Aspergillus oryzae in solid-state culture and construction of the new production system

タンパク質工場としての糸状菌の高度利用に関する基礎的研究 固体培養環境下でのタンパク質生産制御系の解析と新規生産システムの構築
Study period:2001 - 2005
Organization (1):
Investigating Researcher (4):
Research overview:
Filamentous fungi of which representative is Aspergillus oryzae have been utilized for production of various enzymes due to high production ability of protein such as several grams from one liter culture. In this project, host Aspergillus (super Aspergillus) which have an ability to produce numerous types of proteins such as secretary, vacuolar and cellular proteins in culture, by alteration of cellular polarity, intracellular protein transport and cellular surface structures at the molecular levels as well as by analyzing high production ability of protein in Aspergillus using cellular and molecular biological studies. On the other hand, Aspergillus has been known to produce extracellular proteins more efficiently in solid-state cultures compared to in liquid cultures. In this study, the mechanism for this phenomenon is identified using up-to-date molecular biological techniques. Taken together, the system in which secretary, vacuolar and cellular proteins can be efficiently produced in the culture media by liquid culture of which control in fermentation process is easy, is developed.
Keywords (6):
<i>Aspergillus oryzae</i> ,  protein production ,  cell factory ,  control of gene expression ,  post-translational modification ,  protein secretion mechanism
Project name: The basic research on advanced utilization of <i>Aspergillus oryzae</i> as protein production cell factory.
Project Organization (1):
  • Bio-oriented Technology Research Advancement Institute (BRAIN)
Researcher representative of the project  (1):
  • KITAMOTO Katsuhiko
Research program: Promotion of Basic Research Activities for Innovative Biosciences
Ministry with control over the research :
Ministry of Agriculture, Forestry and Fisheries

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