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J-GLOBAL ID：201002295228176420   Reference number：10A0060246

Miniaturized two-dimensional gel electrophoresis of high-molecular-weight proteins using low-concentration multifilament-supporting gel for isoelectric focusing

等電点電気泳動用に低濃度マルチフィラメント支持ゲルを使用した高分子量蛋白質の小型化二次元ゲル電気泳動

Author (10)： ODAKE Tamao (School of Engineering, The Univ. of Tokyo) ,  SAHEKI Toshihiko (Dep. of Chemistry and Chemical Biology, Gunma Univ.) ,  KAMEZAWA Kazutoshi (Dep. of Chemistry and Chemical Biology, Gunma Univ.) ,  MIYATA Kozue (Dep. of Chemistry and Chemical Biology, Gunma Univ.) ,  TAKIGUCHI Hiromi (School of Engineering, The Univ. of Tokyo) ,  HOTTA Hiroki (Dep. of Chemistry and Chemical Biology, Gunma Univ.) ,  ONDA Koki (Textile Res. Inst. of Gunma) ,  KOJIMA Yasuaki (Mitachi Co. Ltd.) ,  LI Jinxiang (Dep. of Chemistry, Liaoning Normal Univ.) ,  TSUNODA Kin-ichi (Dep. of Chemistry and Chemical Biology, Gunma Univ.)
Material： Journal of Electrophoresis  (J Electrophor)
Volume： 53  Issue： 4  Page： 57-61 (J-STAGE)  Publication year： 2009 
JST Material Number： L5383A  ISSN： 1349-9394  CODEN： JEOLAS  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *fiber ,...
Semi thesaurus term： Electrophoresis, Polyacrylamide Gel ,...

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JST classification (1)： Biophysical research methods  (EA03020V)

Reference (13)：

• OH-ISHI, M. Separation techniques for high-molecular-mass proteins. J Chromatogr B. 2002, 771, 49-66
• HIRABAYASHI, T. Two-Dimensional Gel Electrophoresis of Chicken Skeletal Muscle Proteins with Agarose Gels in the First Dimension. Anal Biochem. 1981, 117, 443-451
• OH-ISHI, M. Preparative two-dimensional gel electrophoresis with agarose gels in the first dimension for high molecular mass proteins. Electrophoresis. 2000, 21, 1653-1669
• OH-ISHI, M. 2-D PAGE of high-molecular-mass proteins. Proteomics Protocols Handbook. 2005, 145-149
• LI, J. A new isoelectric focusing system for two-dimensional gel elecreophoresis using a low-concentration polyacrylamide gel supported by a loose multifilament string. Anal Sci. 2004, 20, 1673-1679

Terms in the title (8)： 等電点電気泳動 ,  低濃度 ,  マルチフィラメント ,  支持 ,  ゲル ,  高分子量 ,  蛋白質 ,  二次元ゲル電気泳動