Art

J-GLOBAL ID：201002295810385041   Reference number：10A0239666

Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner

融合パートナーとしてシリカ結合Siタグを用いた組換蛋白質の1段階アフィニティ精製

Author (6)： IKEDA Takeshi (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  IKEDA Takeshi (Res. Inst. for Nanodevice and Bio Systems, Hiroshima Univ., 1-4-2 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, JPN) ,  NINOMIYA Ken-ichi (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  HIROTA Ryuichi (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  KURODA Akio (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  KURODA Akio (Res. Inst. for Nanodevice and Bio Systems, Hiroshima Univ., 1-4-2 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, JPN)
Material： Protein Expression and Purification  (Protein Expression & Purification)
Volume： 71  Issue： 1  Page： 91-95  Publication year： May. 2010 
JST Material Number： W0282A  ISSN： 1046-5928  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： fusion protein ,  adsorbent ,  *recombinant protein ,  Escherichia coli ,  gene cloning ,  gene expression ,  nucleotide sequence ,  yield ,  polypeptide ,  *bacterial protein ,  purification ,  *affinity chromatography ,  marker ,  *silicon dioxide
Semi thesaurus term： *silica ,  tag ,  *Staphylococcal Protein A

JST classification (3)： Biochemical analytical methods  (EA03030G) ,  Proteins and peptides in general  (EB03010N) ,  Gene manipulation  (EC02050B)

Terms in the title (8)： 融合 ,  パートナー ,  シリカ ,  Si ,  タグ ,  組換蛋白質 ,  段階 ,  アフィニティ精製