Art

J-GLOBAL ID：201102206204755938   Reference number：11A0601220

The silica-binding Si-tag functions as an affinity tag even under denaturing conditions

変性条件下でもアフィニティー標識として機能するシリカ結合Si標識

Author (9)： IKEDA Takeshi (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  IKEDA Takeshi (Res. Inst. for Nanodevice and Bio Systems, Hiroshima Univ., 1-4-2 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, JPN) ,  MOTOMURA Kei (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  MOTOMURA Kei (Res. Inst. for Nanodevice and Bio Systems, Hiroshima Univ., 1-4-2 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, JPN) ,  AGOU Yuuya (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  ISHIDA Takenori (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  HIROTA Ryuichi (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  KURODA Akio (Dep. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Univ., 1-3-1 Kagamiyama ...) ,  KURODA Akio (Res. Inst. for Nanodevice and Bio Systems, Hiroshima Univ., 1-4-2 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8527, JPN)
Material： Protein Expression and Purification  (Protein Expression & Purification)
Volume： 77  Issue： 2  Page： 173-177  Publication year： Jun. 2011 
JST Material Number： W0282A  ISSN： 1046-5928  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： denaturation ,  *affinity labeling ,  silicon ,  *purification ,  inclusion body ,  *recombinant protein ,  Escherichia coli ,  gene expression ,  fusion protein ,  molecular model ,  ultrasonic application ,  solubilization ,  protein ,  carboxylic acid ,  nitrogen heterocyclic compound ,  coenzyme ,  urea compound ,  sulfur heterocyclic compound ,  *silicon dioxide ,  vitamin B family
Semi thesaurus term： polycation ,  *silica ,  Si標識 ,  *affinity purification ,  シリカ結合蛋白質

JST classification (1)： Gene expression  (EC02040Q)

Substance index (2)： Biotin ,  Urea

Terms in the title (5)： 変性 ,  アフィニティー標識 ,  シリカ ,  Si ,  標識