J-GLOBAL ID:201110055473073013   Research Resource code:5000000349 Update date:Nov. 24, 2004

Sexing of Porcine Embryo by In Situ Hybridization Using Chromosome Y-and I-Specific DNA Probes

Yおよび1番染色体特異的DNAプローブを用いたIn situハイブリダイゼーション法によるブタ胚の雌雄判別と産み分け
Owning Organization:
Contact: KAWARASAKI Tatsuo
Resource classification: Bibliographic database (Bibliography, Abstract, Full text)
We determined sex by treating the whole embryos in various
stages from three-cell embryos to blastocyst in extending
stage. As a result, detection of the signal depended on the
raising stage of embryos, and the sex could be determined
in 97.4% (114/117) of embryos which are in later stages than
32-cell stage. We also extracted a part of cell from
blastocyst and determined the sex.
The sex could be determined in 91.4% (85/93) embryos and
the survival rate of embryos was 92.3% (60/65) at 24 hours
after biopsy. Examining the methods of operation, we
transplanted the embryos which were determined sex into
female pigs whose estrus cycle were synchronized. In the
4th examination, a pig on which 22 male embryos had been
transplanted got pregnant, and was delivered nine normal
male pigs.

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