Rchr
J-GLOBAL ID:201201098932605000
Update date: May. 23, 2024
Shiroishi Mitsunori
シロイシ ミツノリ | Shiroishi Mitsunori
Affiliation and department:
Job title:
Associate Professor
Homepage URL (1):
https://shiroishimitsunori.wixsite.com/shiroishilab-jp
Research field (4):
Biophysics
, Applied biofunctional and bioprocess engineering
, Functional biochemistry
, Structural biochemistry
Research keywords (7):
receptor, antibody, immunology, G-protein coupled receptor, membrane protein
, protein engineering, antibody engineering
, protein engineering
, immunoreceptor
, 構造解析
, 抗体
, GPCR
Research theme for competitive and other funds (13):
- 2019 - 2022 Elucidation of the molecular recognition of stereotype rheumatoid factor.
- 2015 - 2018 Creation of the membrane-protein-particle antigen for the effective production of a crystallization-facilitator antibody
- 2014 - 2018 Establishment of research foundation on "objective pain assessment" using animal models suffering from neuropathic pain
- 2015 - 2017 Fundamental Study on Preparations for Therapeutic Antibodies with Less Productions of Anti-Drug Antibodies
- 2013 - 2017 Development of the strategy for creating stabilized GPCR variants for large-scale preparation
- 2013 - 2016 Crystallization of Angiotensin receptor type 2 with its specific antibody
- 2011 - 2014 Study on the development of molecules for depressing neuropathic pain based on tertiary structure of human P2X4 receptor
- 2011 - 2012 Elucidating of inhibition mechanism of anti-histamines for the histamine receptor by X-ray crystallography.
- 2011 - 2012 Development of Fab molecule with the stable scaffold for next generation
- 2010 - 2012 Crystallization of GPCR and its specific antibody
- 2009 - 2011 Elucidation of the transport mechanism of a secondary active transporter, Mhp1.
- 2005 - 2006 蛋白質間相互作用と立体構造解析に基づく免疫細胞受容体LIR/ILT群の機能解明
- 2000 - 2002 構造情報を基盤とした蛋白性抗原と抗体間相互作用の精密解析
Show all
Papers (60):
-
Nakagawa F, Kikkawa M, Chen S, Miyashita Y, Hamaguchi-Suzuki N, Shibuya M, Yamashita S, Nagase L, Yasuda S, Shiroishi M, Senda T, Ito K, Murata T, Ogasawara S. Anti-nanodisc antibodies specifically capture nanodiscs and facilitate molecular interaction kinetics studies for membrane protein. Scientific Reports. 2023. 13
-
Suzuki M, Fujimori H, Wakatsuki K, Manaka Y, Asai H, Hyodo M, Matsuno Y, Kusumoto-Matsuo R, Shiroishi M, Yoshioka KI. Genome destabilization-associated phenotypes arising as a consequence of therapeutic treatment are suppressed by Olaparib. PLoS One. 2023. 18. 1
-
Taisei Yamamoto, Satoshi Yasuda, Rinshi S. Kasai, Ryosuke Nakano, Simon Hikiri, Kanna Sugaya, Tomohiko Hayashi, Satoshi Ogasawara, Mitsunori Shiroishi, Takahiro K. Fujiwara, et al. A methodology for creating mutants of G-protein coupled receptors stabilized in active state by combining statistical thermodynamics and evolutionary molecular engineering. Protein Science. 2022. 31. 10
-
Chinatsu Shinozaki, Keita Kohno, Mitsunori Shiroishi, Daisuke Takahashi, Yu Yoshikawa, Yoshito Abe, Kenji Hamase, Makoto Nakakido, Kohei Tsumoto, Kazuhide Inoue, et al. Improvement of the affinity of an anti-rat P2X4 receptor antibody by introducing electrostatic interactions. Scientific reports. 2022. 12. 1. 131-131
-
Structural and Functional Basis for LILRB Immune Checkpoint Receptor Recognition of HLA-G isoforms. 2020. 38. 35-36
more...
MISC (39):
-
白石充典, 松原直紀, 安田賢司, 齊藤秀俊, 津田誠, 木下正弘, 村田武士, 植田正. Stabilization and large-scale preparation of P2Y12 receptor using theoretical prediction method and Saccharomyces cerevisiae expression system. 日本分子生物学会年会プログラム・要旨集(Web). 2021. 44th
-
扇谷和希, 佐藤愼吾, 鈴木七緒, 安田賢司, 白石充典, 木下正弘, 村田武士. メラトニン1A受容体の高発現・熱安定化改変体の創出. 日本生体エネルギー研究会討論会講演要旨集. 2018. 44th
-
中野僚介, 肥喜里志門, 肥喜里志門, 安田賢司, 安田賢司, 白石充典, 木下正弘, 村田武士. GPCR活性型変異体スクリーニング系の構築. 日本生体エネルギー研究会討論会講演要旨集. 2018. 44th
-
豊田洋輔, 森本和志, 寿野良二, 関口雄介, 山下恵太郎, 平田邦夫, 安田賢司, 安田賢司, 白石充典, 堀田韻虹, et al. 抗体を用いたプロスタグランジン受容体のX線結晶構造解析. GPCR研究会プログラム・抄録集. 2016. 13th
-
藤田玲奈, 斎藤貴士, 前仲勝実, 林いづみ, 白石充典, 阿部義人, 植田正. マウスリゾチームにおける部位特異的なアスパラギン残基のラセミ化機構の解析. 日本薬学会九州支部大会講演要旨集. 2013. 30th. 159
more...
Books (10):
-
2022年度版 基礎工学実験
学術図書出版 2022 ISBN:9784780610062
-
膜タンパク質工学ハンドブック
株式会社エヌ・ティ・エス 2020 ISBN:9784860435370
-
Esentialタンパク質科学
南江堂 2016
-
津本浩平、植田正、前仲勝実監訳「Esentialタンパク質科学」
南江堂 2016
-
進化を続ける構造生物学
化学同人 2014
more...
Lectures and oral presentations (94):
-
出芽酵母を利用したヒスタミンH3受容体改変体のシグナル伝達の評価
(第45回日本分子生物学会年会 2022)
-
リウマトイド因子RF-SJ1及びM11の分子認識様式の解明に向けたFv-claspの調製
(第45回日本分子生物学会年会 2022)
-
TEVプロテアーゼ変異体とGFP_nanobodyを利用したヒスタミンH3受容体の精製法の確立
(第45回日本分子生物学会年会 2022)
-
Saccharomyces cerevisiaeを用いたヒト由来ヒスタミンH4受容体の安定化
(第45回日本分子生物学会年会 2022)
-
出芽酵母発現系におけるヒスタミンH3受容体のシグナル活性回復変異の評価
(生物環境イノベーション研究部門・公開シンポジウム 2022)
more...
Education (3):
- 2000 - 2003 Tohoku University Graduate School of Engineering
- 1998 - 2000 Tohoku University Graduate School of Engineering
- 1994 - 1998 Tohoku University
Professional career (1):
Work history (6):
Committee career (1):
- 2022/04/01 - 2026/03/31 PF-UAタンパク質結晶構造解析ユーザーグループ 幹事
Awards (5):
- 2017/03 - 第28回加藤記念研究助成 優秀賞
- 2013/05 - 日本生化学会九州支部会 学術奨励賞
- 2012/04 - 文部科学大臣表彰若手科学者賞
- 2001/08 - Poster award
- 2001/08 - Finn Wold travel award
Association Membership(s) (3):
THE PHARMACEUTICAL SOCIETY OF JAPAN
, PROTEIN SCIENCE SOCIETY OF JAPAN
, THE JAPANESE BIOCHEMICAL SOCIETY
Return to Previous Page