Art

J-GLOBAL ID：201202247795126956   Reference number：12A0273944

Further Stabilization of Leu¹⁵⁵ Mutant Thermolysins by Mutation of an Autodegradation Site

自己分解部位の変異によるLeu¹⁵⁵変異体サーモリシンの更なる安定化

Author (4)： MATSUMIYA Yoshiki (Ritsumeikan Univ., Dep. of Biotechnology, Fac. of Life Sciences, Nojihigashi, 525-8577, Kusatsu, Shiga-ken, JPN) ,  MURATA Naoko (Ritsumeikan Univ., Dep. of Biotechnology, Fac. of Life Sciences, Nojihigashi, 525-8577, Kusatsu, Shiga-ken, JPN) ,  INOUYE Kuniyo (Kyoto Univ., Div. of Food Sci. and Biotechnology, Graduate School of Agriculture, Sakyo-ku, 606-8502, Kyoto, JPN) ,  KUBO Motoki (Ritsumeikan Univ., Dep. of Biotechnology, Fac. of Life Sciences, Nojihigashi, 525-8577, Kusatsu, Shiga-ken, JPN)
Material： Applied Biochemistry and Biotechnology. Part A. Enzyme Engineering and Biotechnology  (Applied Biochemistry and Biotechnology)
Volume： 166  Issue： 3  Page： 735-743  Publication year： Feb. 2012 
JST Material Number： E0344C  ISSN： 0273-2289  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Thesaurus term： *thermolysin ,  autolysis ,  mutant ,  *site-directed mutagenesis ,  *thermal stability ,  amino acid residue ,  temperature dependence ,  *Bacillus ,  proteinase ,  higher order structure ,  gene expression ,  stabilization

JST classification (3)： Enzyme physiology  (EG03052C) ,  Enzyme in general  (EB09010D) ,  Molecular genetics in general  (EC02010J)

Terms in the title (5)： 自己分解 ,  変異 ,  変異体 ,  サーモリシン ,  安定化