Art

J-GLOBAL ID：201302272045594178   Reference number：13A1401080

A NADPH-dependent (S)-imine reductase (SIR) from Streptomyces sp. GF3546 for asymmetric synthesis of optically active amines: purification, characterization, gene cloning, and expression

光学活性アミンの不斉合成に対しStreptomyces sp.GF3546由来NADPH依存性(S)-イミンレダクターゼ(SIR):精製,特性化,遺伝子クローニング,及び発現

Author (6)： MITSUKURA Koichi (Gifu Univ., Dep. of Biomolecular Sci., Fac. of Engineering, 1-1 Yanagido, 501-1193, Gifu, JPN) ,  KURAMOTO Tatsuya (Gifu Univ., Dep. of Biomolecular Sci., Fac. of Engineering, 1-1 Yanagido, 501-1193, Gifu, JPN) ,  YOSHIDA Toyokazu (Gifu Univ., Dep. of Biomolecular Sci., Fac. of Engineering, 1-1 Yanagido, 501-1193, Gifu, JPN) ,  KIMOTO Norihiro (Daicel Corp., Corporate Res. Center, R&D Management, 1239 Shinzaike, Aboshi-ku, 671-1283, Himeji-shi, Hyogo, JPN) ,  YAMAMOTO Hiroaki (Daicel Corp., Green Product Dev. Center, R&D Management, 1-1 Shinko-cho, 944-8550, Niigata, Myoko-shi, JPN) ,  NAGASAWA Toru (Gifu Univ., Dep. of Biomolecular Sci., Fac. of Engineering, 1-1 Yanagido, 501-1193, Gifu, JPN)
Material： Applied Microbiology and Biotechnology  (Appl Microbiol Biotechnol)
Volume： 97  Issue： 18  Page： 8079-8086  Publication year： Sep. 2013 
JST Material Number： H0764A  ISSN： 0175-7598  CODEN： EJABDD  Document type： Article
Article type： 原著論文  Country of issue： Germany, Federal Republic of (DEU)  Language： ENGLISH (EN)

Thesaurus term： *asymmetric synthesis ,  optical activity ,  amine ,  *Streptomyces ,  *reductase ,  imine ,  purification ,  characterization ,  gene cloning ,  *gene expression ,  dimer ,  cell-free system ,  stereoselectivity ,  reduction(reaction) ,  enzyme activity ,  Escherichia coli ,  substrate specificity ,  enzymatic synthesis ,  coenzyme ,  adenine nucleotide ,  nicotinamide mononucleotide
Semi thesaurus term： enantioselectivity ,  characterization

JST classification (4)： Enzyme physiology  (EG03052C) ,  Enzyme in general  (EB09010D) ,  Stereochemistry of organic reaction  (CF02130V) ,  Gene expression  (EC02040Q)

Substance index (1)： NADPH

Terms in the title (12)： 光学活性 ,  アミン ,  不斉合成 ,  Streptomyces ,  NADPH ,  依存性 ,  イミンレダクターゼ ,  SIR ,  精製 ,  特性化 ,  遺伝子クローニング ,  発現