Research keywords (7):
Polymerization and depolymerization dynamics of actin
, Cytoskeleton
, Cell motility
, MreB
, Foce generation
, protein theymodynamics
, Biophysics
Research theme for competitive and other funds (5):
2020 - 2023 TIRF visualization of the polymerization dynamics of bacterial actin cytoskeleton, MreB and A22 driven disruption mechanism
2019 - 2021 Visualization of fluorescent labeled proteins required for helical-shaped bacterial swimming motility and the force propagation
2017 - 2020 ゲルゾリン様タンパク質によるアクチン繊維切断機構の解明
2015 - 2017 細胞伸張制御におけるアクチン重合調節タンパク質CPの役割
2001 - 2002 単一アクチンフィラメント直視による重合・脱重合ダイナミクス機構の解明
Papers (35):
Daichi Takahashi, Hana Kiyama, Hideaki Matsubayashi, Ikuko Fujiwara, Makoto Miyata. A highly active bacterial actin actuates the polymerization of another isoform essential for swimming motility ofSpiroplasma. 2024
Daichi Takahashi, Makoto Miyata, Ikuko Fujiwara. Assembly properties of bacterial actin MreB involved in Spiroplasma swimming motility. The Journal of biological chemistry. 2023. 299. 6. 104793-104793
Kazuto Tsukita, Manabu Kitamata, Hiroka Kashihara, Tomoki Yano, Ikuko Fujiwara, Timothy F Day, Tatsuya Katsuno, Jaewon Kim, Fumiko Takenaga, Hiroo Tanaka, et al. Phase separation of an actin nucleator by junctional microtubules regulates epithelial function. Science advances. 2023. 9. 7. eadf6358
Daichi Takahashi, Ikuko Fujiwara, Makoto Miyata. Purification and ATPase Activity Measurement of Spiroplasma MreB. Methods in molecular biology (Clifton, N.J.). 2023. 2646. 359-371
Yusuke Kanematsu, Akihiro Narita, Toshiro Oda, Ryotaro Koike, Motonori Ota, Yu Takano, Kei Moritsugu, Ikuko Fujiwara, Kotaro Tanaka, Hideyuki Komatsu, et al. Structures and mechanisms of actin ATP hydrolysis. Proceedings of the National Academy of Sciences of the United States of America. 2022. 119. 43. e2122641119
武田修一, 兼松佑典, 成田哲博, 田中康太郎, 小池亮太郎, 太田元規, 鷹野優, 森次圭, 藤原郁子, 渡邉信久, et al. F-form actin structure at an atomic resolution: how the molecular assembly and the ATP hydrolysis are coupled. 日本物理学会講演概要集(CD-ROM). 2020. 75. 1
Fujiwara, I, S Suetsugu, S Uemura, H Miki, T Takenawa, S Ishiwata. Direct observation of branching in actin filaments triggered by Arp2/3 complex. BIOPHYSICAL JOURNAL. 2002. 82. 1. 385A-385A
Fujiwara, I, S Takahashi, H Tadakuma, T Funatsu, S Ishiwata. Microscopic analysis of polymerization process of single actin filaments. BIOPHYSICAL JOURNAL. 2001. 80. 1. 94A-94A
Books (1):
一分子生物学
同仁化学出版 2014
Lectures and oral presentations (78):
Inhibitory mechanisms of Cytochalasin D in actin dynamics at single filament level through TIRF observation
(American Society of Cell Biology 2024)
Crosstalk of two bacterial actins essential for Spiroplasma swimming
(American Society for Cell Biology 2024)
Purification and characterization of bacterial actin MreB1: a component of swimming machinery in Spiroplasma
(The 61st Annual Meeting of the Biophysical Society of Japan 2023)
Measurement of motor protein ATPase and binding dissociation by QCM
(The 61st Annual Meeting of the Biophysical Society of Japan 2023)
Understanding the orientation of actin subunits by conjugating with jasplakinolide or phalloidin through FRET observation
(The 61st Annual Meeting of the Biophysical Society of Japan 2023)
1998 - 2003 Graduate school of science, Waseda University, Japan Graduate School, Division of Science and Engineering Dept. of Physics
- 1998 Waseda University Faculty of Science and Engineering Dept of Applied Physics
Professional career (1):
博士(理学) (早稲田大学)
Work history (8):
2022/04 - 現在 Nagaoka University of Technology Graduate School of Engineering, Department of Materials Sciences and Bioengineering Assoc. Prof.
2021/04 - 2022/03 Nagaoka University of Technology Graduate School of Engineering Department of Bioengineering Assoc. Prof.
2019/04 - 2021/03 Osaka City University Graduate School of Science Biology and Geosciences Course Assistant Professor
2015/01/01 - 2019/03/31 Nagoya Institute of Technology Reasearch Admistration Office University Research Administrator (Research Assistant Professor)
2014/04/01 - 2014/12/31 Institute for Integrated Cell-Material Sciences, Kyoto University Assistant Professor
2006/09/06 - 2014/03/31 National Institutes of Health, USA Researcher
2004/08/01 - 2006/09/05 Yale University Molecular Cellular and Developmental Biology Researcher
Nagoya Institute of Technology Reasearch Admistration Office University Research Administrator (Research Assistant Professor)
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Committee career (5):
2022/01 - 2024/12 The Biophysical Society of Japan Editorial board member
2021/01 - 2021/12 Biophysical society of Japan 分野別専門委員 (アクチン)
2020/01 - 2020/12 Biophysical society of Japan 分野別専門委員 (細胞骨格)
2018/01 - 2018/12 名古屋工業大学ダイバーシティ推進センター スーパーバイザー
2016/11 - 2017/11 American Society For Cell Biology (ASCB) 名古屋工業大学 アンバサダー
Awards (1):
2018/10 - Faculty of 1000 Ltd | Science Navigation Group Recommended in F1000Prime as being of special significance in its field by F1000 Latrunculin A accelerates actin filament depolymerization in addition to sequestering actin monomers
Association Membership(s) (4):
The Molecular Biology Society of Japan
, The American Society for Cell Biology
, Japan Society for Cell Biology
, The Biophysical Society of Japan