Rchr
J-GLOBAL ID:201401071053492412   Update date: Apr. 04, 2024

Yasukawa Kiyoshi

ヤスカワ キヨシ | Yasukawa Kiyoshi
Affiliation and department:
Research field  (1): Applied biochemistry
Research theme for competitive and other funds  (8):
  • 2022 - 2025 高機能等温核酸増幅酵素の創製と構造解析および病原体の現場での迅速検出への応用
  • 2019 - 2023 温度勾配ゲル電気泳動によるHIV-1逆転写酵素遺伝子変異の迅速検出
  • 2018 - 2021 Development and application of genome profiling system featuring high-speed and large image processing
  • 2015 - 2018 Elucidation of the mecahsim of thermostabilization of thermostable reverse transcriptase
  • 2009 - 2011 Evaluation of cDNA synthesis and RNA amplification with highly efficient reverse transcriptases and expansion of their use
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Papers (198):
  • Tomoka Nakamura, Teisuke Takita, Kohei Kuwata, Kimihiko Mizutani, Bunzo Mikami, Satoshi Nakamura, Kiyoshi Yasukawa. Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR. Scientific reports. 2024. 14. 1. 7767-7767
  • Kenta Morimoto, Kevin Maafu Juma, Masaya Yamagata, Teisuke Takita, Kenji Kojima, Koichiro Suzuki, Itaru Yanagihara, Shinsuke Fujiwara, Kiyoshi Yasukawa. Increase in the solubility of uvsY using a site saturation mutagenesis library for application in a lyophilized reagent for recombinase polymerase amplification. Molecular biology reports. 2024. 51. 1. 367-367
  • Saori Ueshima, Mizuki Yasumoto, Yuto Kitagawa, Kaho Akazawa, Teisuke Takita, Keisuke Tanaka, Shunji Hattori, Kimihiko Mizutani, Bunzo Mikami, Kiyoshi Yasukawa. Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses. FEBS letters. 2023. 597. 19. 2473-2483
  • Kevin Maafu Juma, Eisuke Inoue, Kengo Asada, Wakao Fukuda, Kenta Morimoto, Masaya Yamagata, Teisuke Takita, Kenji Kojima, Koichiro Suzuki, Yukiko Nakura, et al. Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae. Journal of bioscience and bioengineering. 2023
  • Takeaki Ikeuchi, Mizuki Yasumoto, Teisuke Takita, Keisuke Tanaka, Masashi Kusubata, Osamu Hayashida, Shunji Hattori, Kimihiko Mizutani, Bunzo Mikami, Kiyoshi Yasukawa. Crystal structure of Grimontia hollisae collagenase provides insights into its novel substrate specificity toward collagen. The Journal of biological chemistry. 2022. 298. 8. 102109-102109
more...
MISC (70):
Books (5):
  • Essential Protein Engineering
    Kodansha 2018
  • アスパルテームの合成
    酵素利用技術大系(小宮山眞監修)p. 632-636、株式会社エヌ・ティー・エス、東京、2010 2010
  • サーモライシンの応用と高機能化
    産業酵素の応用技術と最新動向(井上國世、監修)p. 58-68、シーエムシー出版、東京、2009 2009
  • 核酸増幅法
    産業酵素の応用技術と最新動向(井上國世、監修)p. 194-202、シーエムシー出版、東京、2009 2009
  • Development of cDNA synthesis technology by a combination of novel enzymes
Lectures and oral presentations  (4):
  • Characterization of Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptase
    (RNase H 2012 2012)
  • タンパク質工学による逆転写酵素の耐熱化(シンポジウム:タンパク質工学による耐熱化酵素創製の新しい戦略)
    (2011年度日本農芸化学会大会 2011)
  • 逆転写酵素の活性と熱安定性(シンポジウム:核酸に関わる酵素の化学)
    (2009年度日本農芸化学会大会 2009)
  • 基質特異性を改変させたことにより自己活性化能を消失したプロテアーゼの大腸菌での生産(タンパク質発現手法の最適化-より良い機能性タンパク質を求めて)
    (2009年度日本農芸化学会大会 2009)
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